Gastrointestinal Involvement in m.3243A>G-associated MELAS

Abstract

To the Editor We read with interest the article by Suzuki et al. about three patients harbouring the m.3243A>G mutation manifesting as mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome and intestinal pseudo-obstruction (Table) (1). We have several comments and concerns. Table. A Comparison of the Three Described Patients. P1 P2 P3 Sex f f f Height (cm) 140 np 147.5 Weight (kg) 25.1 np 47.8 Age at onset (y) 10 12 39 First manifestation SLE SLE Diabetes Presentation vomiting, headache, multiple strokes staggering gait, cerebral atrophy, headache, vomiting bradykinesia, rigidity, multiple strokes seizures, forced crying/laughing, blurring, seizures, bloating abdominal pain, abdominal pain, vomiting, dystonia bloating muscle weakness, hypoacusis, myopathy gait ataxia, neuropathy, increased reflexes, cerebral atrophy, hot cross bun sign, urinary retention NOO involved 4 2 5 Creatin kinase (U/L) 105 np 16 Lactate (mg/dL) 14.7 np 19.4 Degree of progression np np np Age at death (y) 23 22 54 Cause of death Aspiration pneumonia Renal failure Aspiration pneumonia Genetic cause m.3243A>G m.3243A>G m.3243A>G Sampled tissue blood blood blood Heteroplasmy rate np np np NOO: number of organs, SLE: stroke-like episode, np: not provided Mitochondrial disorders (MIDs) may accompany neuropathy of the peripheral nerves, including affection of the autonomic fibers (2). As such, intestinal pseudo-obstruction can occur due to autonomic neuropathy. Were there indications for autonomic neuropathy in any of your cases, such as increased sensitivity to light, dry mouth, dry eyes, orthostasis, abnormal heart rate variability, obstipation, urinary dysfunction, impotence, or dry skin? Were instrumental investigations carried out to search for autonomic neuropathy? The hot cross bun sign is a non-specific morphological feature on magnetic resonance imaging (MRI) and has not only been described in multisystem atrophy-C, spinocerebellar ataxia-2 (SCA2) and SCA3 but also in Alzheimer’s disease, natalizumab-induced, progressive multifocal leucencephalopathy, neurosarcoidosis, late-onset SCA11, single large-scale mtDNA deletions, paraneoplastic syndromes, SCA34, leptomeningeal metastasis, SCA23, HIV-related multifocal leucoencephalopathy, brainstem stroke, familial amyotrophic lateral sclerosis, cerebrotendineous xanthomatosis, Parkinson syndrome, and Creutzfeld-Jacob disease. Atrophy of the cerebellum or brainstem is a frequent finding in MIDs and may even be the dominant feature of the phenotype (3). Cerebellar atrophy has been reported in Leigh syndrome, Kearns-Sayre syndrome, Myoclonus epilepsy associated with ragged-red fibers (MERRF) syndrome, external ophthalmoplegia, Neuropathy, Ataxia, and Retinitis Pigmentosa (NARP) syndrome, Leukoencephalopathy and Brainstem and Spinal cord involvement and Lactate elevation (LBSL), ponto-cerebellar hypoplasia-6, and non-specific mitochondrial multiorgan disorder syndrome (MIMODS). The causes of phenotypic heterogeneity within or between families are versatile and include a variable heteroplasmy rate in different individuals (4) as well as haplotype, mtDNA-polymorphisms, and nuclear mutations. Work-up for phenotypic heterogeneity should also include determination of the heteroplasmy rate in different affected and non-affected tissues, such as hair follicles, buccal mucosa, skin fibroblasts, muscle cells, and urine bladder cells. Gastrointestinal compromise is a frequent manifestation in MIDs and may not only include pseudoobstruction but also poor appetite, gastroesophageal sphincter dysfunction, constipation, dysphagia, vomiting, gastroparesis, diarrhoea, pancreatitis, and hepatopathy (5). Rare gastrointestinal manifestations of MIDs include dry mouth, paradontosis, tracheoesophageal fistula, stenosis of the duodeno-jejunal junction, atresia or imperforate anus, liver cysts, pancreas lipomatosis, pancreatic cysts, congenital stenosis or obstruction of the gastrointestinal tract, recurrent bowel perforations with intra-abdominal abscesses, postprandial abdominal pain, diverticulosis, and pneumatosis coli (5). Were any of these manifestations present in any of the patients? Overall, this interesting case study would profit from the determination of the heteroplasmy rates in different tissues, prospective investigations for MIMODS, investigations for autonomic neuropathy, and from a more extensive family history. Gastrointestinal abnormalities should be regarded as typical manifestations of MIMODS. The authors state that they have no Conflict of Interest (COI).