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      Genetic variability in Aegorhinus superciliosus (Coleoptera: Curculionidae) populations in Chilean Maytenus boaria (Celastrales: Celastraceae) Translated title: Variabilidad genética en poblaciones de Aegorhinus superciliosus (Coleoptera: Curculionidae), en Maytenus boaria (Celastrales: Celastraceae) de Chile


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          Abstract The raspberry weevil (Aegorhinus superciliosus) is a curculionid native of Chile and part of Argentina. This insect is considered to be a pest to both European hazel (Corylus avellana) and blueberry (Vaccinium corymbosum) fruit plantations in south-central Chile and has caused considerable economic damage to the local fruit industry. The aim of this study was to evaluate the genetic variability within A. superciliosus populations associated with Maytenus boaria. Adult A. superciliosus specimens were collected from Máfil, Futrono, La Unión and Frutillar populations. Their classification and storage was carried out at the Animal Biotechnology Research Laboratory (LINBA) of the Universidad de La Frontera, Temuco, Chile. Molecular analysis was assessed through ISSR (Inter Simple Sequence Repeats). Results showed high genetic similarities across all studied populations. A positive correlation was observed between genetic variability and geographic distribution. The southernmost populations (Futrono, La Unión and Frutillar) expressed high interspecific genetic similarities, associated with the abundance of M. boaria, which allows for genetic flow throughout the studied area. The low level of genetic variability in A. superciliosus populations in southern Chile is likely to be correlated with the large number of hosts found in agricultural and forest ecosystems which have the capacity to colonize extensive areas in this part of the country. These conditions contribute to an increase in the genetic flux of A. superciliosus, thus transforming this insect into one of the most significant and most harmful blueberry pests in Chile.

          Translated abstract

          Resumen El cabrito del frambueso (Aegorhinus superciliosus), es un curculiónido nativo de Chile y parte de Argentina. En Chile, habita la zona centro-sur y es considerado una plaga importante, ya que afecta a las plantaciones de frutales arbustivos de avellano europeo (Corylus avellana) y arándano (Vaccinium corymbosum), causando cuantiosos daños económicos a la industria frutícola de la zona. El objetivo de este estudio fue analizar la variabilidad genética de las poblaciones de A. superciliosus asociadas a Maytenus boaria. Ejemplares adultos de A. superciliosus fueron recolectados en las poblaciones de Máfil, Futrono, La Unión y Frutillar. Su clasificación y almacenamiento se realizó en el Laboratorio de Investigación en Biotecnología Animal (LINBA) de la Universidad de La Frontera, Temuco, Chile. El análisis molecular se realizó mediante Marcadores Intermicrosatelitales (ISSR). Los resultados arrojaron una alta similitud génica entre todas las poblaciones de estudio. Se observó una correlación positiva entre la variabilidad genética y la distribución geográfica. Las poblaciones distribuidas más al sur (Futrono- La Unión- Frutillar) presentaron entre si una elevada similitud génica, situación asociada a la abundante presencia de M. boaria el cual permite el flujo génico a lo largo del territorio donde se realizó el estudio. El bajo nivel de variabilidad genética en las poblaciones de A. superciliosus del sur de Chile, está probablemente asociado al gran número de huéspedes encontrados en los ecosistemas agrícolas y forestales, que tienen la capacidad de colonizar grandes áreas en esta zona del país. Estas condiciones contribuyen a incrementar el flujo génico de A. superciliosus, transformando a este insecto en la mayor y más peligrosa plaga de arándanos en Chile.

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          Most cited references36

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          The magnitudes of the systematic biases involved in sample heterozygosity and sample genetic distances are evaluated, and formulae for obtaining unbiased estimates of average heterozygosity and genetic distance are developed. It is also shown that the number of individuals to be used for estimating average heterozygosity can be very small if a large number of loci are studied and the average heterozygosity is low. The number of individuals to be used for estimating genetic distance can also be very small if the genetic distance is large and the average heterozygosity of the two species compared is low.
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            Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification.

            Simple sequence repeats (SSR), or microsatellites, are ubiquitous in eukaryotic genomes. Here we demonstrate the utility of microsatellite-directed DNA fingerprinting by polymerase chain reaction (PCR) amplification of the interrepeat region. No sequencing is required to design the oligonucleotide primers. We tested primers anchored at 3' or 5' termini of the (CA)n repeats, extended into the flanking sequence by 2 to 4 nucleotide residues [3'-anchored primers: (CA)8RG, (CA)8RY, and (CA)7RTCY; and 5'-anchored primers: BDB(CA)7C, DBDA(CA)7, VHVG(TG)7 and HVH(TG)7T]. Radioactively labeled amplification products were analyzed by electrophoresis, revealing information on multiple genomic loci in a single gel lane. Complex, species-specific patterns were obtained from a variety of eukaryotic taxa. Intraspecies polymorphisms were also observed and shown to segregate as Mendelian markers. Inter-SSR PCR provides a novel fingerprinting approach applicable for taxonomic and phylogenetic comparisons and as a mapping tool in a wide range of organisms. This application of (CA)n repeats may be extended to different microsatellites and other common dispersed elements.
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              Gene Flow in Plant Pathosystems


                Author and article information

                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Revista Colombiana de Entomología
                Rev. Colomb. Entomol.
                Sociedad Colombiana de Entomología (Bogotá, Distrito Capital, Colombia )
                December 2018
                : 44
                : 2
                : 260-265
                [5] Temuco Araucanía orgnameUniversidad de La Frontera Chile ramon.rebolledo@ 123456ufrontera.cl
                [1] Temuco Araucanía orgnameUniversidad de La Frontera Chile luishuala@ 123456gmail.com
                [4] Santiago orgnameMuseo Nacional de Historia Natural Chile mario.elgueta@ 123456mnhn.cl
                [3] Temuco Araucanía orgnameUniversidad de La Frontera Chile luis.salazar.navarrete@ 123456gmail.com
                [2] Temuco Araucanía orgnameUniversidad de La Frontera Chile marco.paredes@ 123456ufrontera.cl

                This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

                : 10 August 2017
                : 26 May 2018
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 48, Pages: 6

                SciELO Colombia

                Basic section

                Cabrito del frambueso,marcadores ISSR,flujo génico,Raspberry weevil,ISSR markers,genetic flow


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