The structural gene for yeast DNA topoisomerase I (TOP1) has been cloned from two
yeast genomic plasmid banks. Integration of a plasmid carrying the gene into the chromosome
and subsequent genetic mapping shows that TOP1 is identical to the gene previously
called MAK1. Seven top1 (mak1) mutants including gene disruptions are viable, demonstrating
that DNA topoisomerase I is not essential for viability in yeast. A 3787-base-pair
DNA fragment including the gene has been sequenced. The protein predicted from the
DNA sequence has 769 amino acids and a molecular weight of 90,020.