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      Lack of Bradykinin-lnduced Smooth Muscle Cell Hyperpolarization despite Heterocellular Dye Coupling and Endothelial Cell Hyperpolarization in Porcine Ciliary Artery


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          In porcine coronary artery, bradykinin-induced endothelium-dependent vasodilatations are associated with simultaneous endothelium as well as endothelium-dependent smooth muscle cell (SMC) hyperpolarizations. In contrast, in porcine ciliary artery bradykinin evokes endothelium-dependent relaxations, but no change in SMC membrane potential. This study addresses the question of whether the lack of bradykinin-induced SMC hyperpolarization is also associated with an absence of endothelial hyperpolarization in porcine ciliary artery. With a microelectrode to impale cells in arterial strips, a 12-mV transient bradykinin-induced hyperpolarization was measured in endothelial cells. Bradykinin evoked no SMC hyperpolarization deep in the media. Only occasionally, a slight 4-mV hyperpolarization could be recorded in some SMC next to the endothelium. The endothelial intracellular injection (through the recording electrode) of the fluorescent tracers, lucifer yellow or ethidium bromide, showed the existence of a heterocellular dye coupling between endothelial cells and SMC. These observations in porcine ciliary artery demonstrate that the lack of bradykinin-induced endothelium-dependent SMC hyperpolarization is not due to an absence of endothelial cell hyperpolarization, but most likely to an insufficient electrotonic propagation space constant from endothelial cells to SMC, despite the presence of a dye coupling between these cells.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          24 September 2008
          : 34
          : 5
          : 344-350
          aDepartment of Zoology and Animal Biology, Sciences III, Geneva University, Geneva, and bLaboratory of Ocular Pharmacology and Physiology, University Eye Clinic Basel, Switzerland
          159243 J Vasc Res 1997;34:344–350
          © 1997 S. Karger AG, Basel

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          Pages: 7
          Research Paper


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