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      Pulmonary Artery Endothelial Dysfunction following Ischemia and Reperfusion of the Rabbit Lung

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          Abstract

          We studied endothelial dysfunction of the rabbit pulmonary artery following in vivo ischemia and reperfusion of the lung, and also investigated the mechanisms of endothelium-dependent relaxation in these arteries. Intrapulmonary arteries were isolated from rabbits subjected to ischemia and reperfusion of one lung. Percent relaxation values of sham-operated (i.e. nonischemic) pulmonary arteries to endothelium-dependent vasodilators acetylcholine (ACh) and A23187 were 72 ± 4 and 65 ± 4%, respectively, while relaxation to the endothelium-independent dilator NaNO<sub>2</sub> was 97 ± 1 %. The relaxation of control artery rings to ACh and A23187 were significantly decreased to 2 ± 1 and 5 ± 4%, respectively, following addition of Nω-nitro- L-arginine methyl ester, while relaxation following treatment with indomethacin or glybenclamide remained normal. Relaxation to NaNO<sub>2</sub> was not altered by pretreatment with any of the above compounds. Thus, pulmonary artery relaxation to the endothelium-dependent dilators ACh and A23187 appears to be mediated by the release of EDRF. Endothelium-dependent relaxation of pulmonary arteries from lungs exposed to 90 min of ischemia and 30 min of reperfusion remained essentially normal, while 90 min of ischemia followed by 60 min of reperfusion resulted in a significant decrease in endothelium-dependent relaxation to A23187 to 37 ± 7% (p < 0.05), whereas the response to ACh was reduced only to 57 ± 3% (not significant). 90 min of ischemia followed by 90 min of reperfusion resulted in significant attenuation of endothelium-dependent relaxation to both ACh (36 ± 4%) and A23187 (33 ± 7%). Intrapulmonary artery rings from rabbits given superoxide dismutase starting 15 min before reperfusion showed an improved relaxation to ACh and A23187(55 ± 5 and 58 ± 11%, respectively, at 90 min of reperfusion). Thus, in vivo ischemia and reperfusion of the rabbit lung results in significant endothelial dysfunction which worsens with increasing reperfusion time. Some of the endothelial dysfunction appears to be related to superoxide radical formation.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1993
          1993
          23 September 2008
          : 30
          : 3
          : 145-153
          Affiliations
          Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa., USA
          Article
          158989 J Vasc Res 1993;30:145–153
          10.1159/000158989
          8518332
          © 1993 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 9
          Categories
          Research Paper

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