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      Matching X-ray beam and detector properties to protein crystals of different perfection

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          Abstract

          Expressions are given to match X-ray data collection facilities to the intrinsic diffraction properties of crystals with different degrees of perfection.

          Abstract

          An analysis is given of the effect of different beam and detector parameters on the sharpness of recorded diffraction features for macromolecular crystals of different quality. The crystal quality parameters include crystal strain, crystal or mosaic block size and mosaic block misorientation. Calculations are given for instrument parameters such as angular resolution of the detector, beam divergence and wavelength bandpass to be matched to the intrinsic diffraction properties from these crystals with the aim of obtaining the best possible data out of each crystal. Examples are given using typical crystal imperfections obtained from the literature for both room-temperature and cryo-cooled crystals. Possible implications for the choice of X-ray source, beamline design, detector specifications, instrument set-up and data processing are discussed, together with the limitations of the approach.

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          Author and article information

          Journal
          J Synchrotron Radiat
          J Synchrotron Radiat
          J. Synchrotron Rad.
          Journal of Synchrotron Radiation
          International Union of Crystallography
          0909-0495
          1600-5775
          01 May 2014
          18 March 2014
          18 March 2014
          : 21
          : Pt 3 ( publisher-idID: s140300 )
          : 537-546
          Affiliations
          [a ]Diamond Light Source Ltd , Harwell Science and Innovation Campus, Didcot OX11 0DE, UK
          Author notes
          Correspondence e-mail: colin.nave@ 123456diamond.ac.uk
          Article
          wa5053 JSYRES S1600577514003609
          10.1107/S1600577514003609
          3998814
          24763643
          fd42dc37-537a-4ab4-8e2d-9dc1c5ca89c3
          © Colin Nave 2014

          This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

          History
          : 14 March 2013
          : 17 February 2014
          Categories
          Research Papers

          Radiology & Imaging
          crystal perfection,synchrotron beam properties,detectors
          Radiology & Imaging
          crystal perfection, synchrotron beam properties, detectors

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