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      Proteomic profiling of peripheral blood and bronchoalveolar lavage fluid in interstitial lung diseases: an explorative study

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          Abstract

          Interstitial lung diseases (ILDs) are a diverse group of disorders, which differ significantly with respect to aetiopathogenesis, clinical, radiological, and pathological picture. Idiopathic pulmonary fibrosis (IPF) and sarcoidosis represent two distinctive examples of ILDs [1].

          Abstract

          Proximity extension assay proves feasible for multiplex analysis of proteins in bronchoalveolar lavage. Small exploratory study found multiple inflammatory proteins that differed between patients with sarcoidosis and idiopathic pulmonary fibrosis. https://bit.ly/3nW14nF

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          Diagnosis of Idiopathic Pulmonary Fibrosis. An Official ATS/ERS/JRS/ALAT Clinical Practice Guideline

          Ganesh Raghu, Martine Remy-Jardin, Jeffrey L Myers … (2018)
          This document provides clinical recommendations for the diagnosis of idiopathic pulmonary fibrosis (IPF). It represents a collaborative effort between the American Thoracic Society, European Respiratory Society, Japanese Respiratory Society, and Latin American Thoracic Society.
          Article 0 comments Cited 1353 times – based on 0 reviews     Review now
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            Homogenous 96-Plex PEA Immunoassay Exhibiting High Sensitivity, Specificity, and Excellent Scalability

            Erika Assarsson, Martin Lundberg, Göran Holmquist … (2014)
            Medical research is developing an ever greater need for comprehensive high-quality data generation to realize the promises of personalized health care based on molecular biomarkers. The nucleic acid proximity-based methods proximity ligation and proximity extension assays have, with their dual reporters, shown potential to relieve the shortcomings of antibodies and their inherent cross-reactivity in multiplex protein quantification applications. The aim of the present study was to develop a robust 96-plex immunoassay based on the proximity extension assay (PEA) for improved high throughput detection of protein biomarkers. This was enabled by: (1) a modified design leading to a reduced number of pipetting steps compared to the existing PEA protocol, as well as improved intra-assay precision; (2) a new enzymatic system that uses a hyper-thermostabile enzyme, Pwo, for uniting the two probes allowing for room temperature addition of all reagents and improved the sensitivity; (3) introduction of an inter-plate control and a new normalization procedure leading to improved inter-assay precision (reproducibility). The multiplex proximity extension assay was found to perform well in complex samples, such as serum and plasma, and also in xenografted mice and resuspended dried blood spots, consuming only 1 µL sample per test. All-in-all, the development of the current multiplex technique is a step toward robust high throughput protein marker discovery and research.
            Article 0 comments Cited 687 times – based on 0 reviews     Review now
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              Statement on sarcoidosis. Joint Statement of the American Thoracic Society (ATS), the European Respiratory Society (ERS) and the World Association of Sarcoidosis and Other Granulomatous Disorders (WASOG) adopted by the ATS Board of Directors and by the ERS Executive Committee, February 1999.

              R Baughman, M Ando, SK Ibrahim … (1999)
              Article 0 comments Cited 490 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): ERJ Open Res
                Journal ID (iso-abbrev): ERJ Open Res
                Journal ID (publisher-id): ERJOR
                Journal ID (hwp): erjor
                Title: ERJ Open Research
                Publisher: European Respiratory Society
                ISSN (Electronic): 2312-0541
                Publication date Collection: January 2021
                Publication date (Electronic): 29 March 2021
                Volume: 7
                Issue: 1
                Electronic Location Identifier: 00489-2020
                Affiliations
                [1 ]Dept of Pneumology and Allergy, Medical University of Lodz, Lodz, Poland
                [2 ]Dept of Medical Sciences: Clinical Physiology, Uppsala University, Uppsala, Sweden
                [3 ]Dept of Medical Sciences: Respiratory Medicine, Sleep and Allergy, Uppsala University, Uppsala, Sweden
                [4 ]Dept of Pathobiology of Respiratory Diseases, Medical University of Lodz, Lodz, Poland
                Author notes
                Andrei Malinovschi, Dept of Medical Sciences: Clinical Physiology, Uppsala University, Akademiska sjukhuset Ing 35, 2tr. Uppsala 75185, Sweden. E-mail: andrei.malinovschi@ 123456medsci.uu.se
                Author information
                https://orcid.org/0000-0002-7210-5552
                https://orcid.org/0000-0003-1287-9289
                https://orcid.org/0000-0002-4098-7765
                Article
                Publisher ID: 00489-2020
                DOI: 10.1183/23120541.00489-2020
                PMC ID: 8005592
                PubMed ID: 33816595
                SO-VID: fd83d348-5abd-44fc-b532-3ff899b0cee7
                Copyright © Copyright ©The authors 2021
                License:

                This version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. For commercial reproduction rights and permissions contact permissions@ersnet.org

                History
                Date received : 10 July 2020
                Date accepted : 03 January 2021
                Funding
                Funded by: Department of Pneumology and Allergy, Medical University of Lodz, Poland
                Funded by: Knut och Alice Wallenbergs Stiftelse, open-funder-registry 10.13039/501100004063;
                Funded by: Hjärt-Lungfonden, open-funder-registry 10.13039/501100003793;
                Categories
                Subject: Original Research Letters
                Subject: 12
                Subject: 13

                Data availability:

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