Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing.

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Abstract

Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

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Journal

Journal ID (iso-abbrev): Parasitol. Int.

Title: Parasitology international

Publisher: Elsevier BV

ISSN (Electronic): 1873-0329

ISSN (Print): 1383-5769

Publication date (Electronic): Feb 2018

Volume: 67

Issue: 1

Affiliations

[1 ] Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College, Japan. Electronic address: kazu_imai@ndmc.ac.jp.

[2 ] Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan.

[3 ] Department of Pediatric Emergency Medicine, Osaka City General Hospital, Osaka, Japan.

[4 ] Department of Plastic Surgery, Osaka City General Hospital, Osaka, Japan.

[5 ] Department of Infectious Diseases, Tokyo Metropolitan Bokutoh General Hospital, Tokyo, Japan.

[6 ] Division of Preparedness and Emerging Infections, Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan.

[7 ] Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College, Japan; Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan.

[8 ] Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan; Department of Microbiology, Saitama Medical University, Saitama, Japan.

[9 ] Department of Computational Biology and Medical Science, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.

Article

Publisher Item ID: S1383-5769(17)30043-0

DOI: 10.1016/j.parint.2017.03.001

PubMed ID: 28288843

SO-VID: fd8973c3-87f3-4ceb-b454-dd08938fc26e

History

Keywords: Leishmania braziliensis,Cutaneous leishmaniasis;,Diagnosis,LAMP;,Leishmania tropica,MinION™

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Keywords: Leishmania braziliensis, Cutaneous leishmaniasis;, Diagnosis, LAMP;, Leishmania tropica, MinION™

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing.

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