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      Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium.

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          Abstract

          Much investigation is needed to understand the underlying molecular mechanisms of iPSC reprogramming and to improve this technology. Lentivirus-mediated iPSC reprogramming remains the most effective method to study human pluripotency reprogramming. iPSC production is more efficient and consistent in the chemically defined medium. Fibroblasts are the most common starting cells for iPSC generation. Here, we provide a detailed protocol for iPSC generation from human fibroblasts using the GFP-expressing lentiviral vectors in the chemically defined medium.

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          Author and article information

          Journal
          Journal ID (iso-abbrev): Methods Mol Biol
          Title: Methods in molecular biology (Clifton, N.J.)
          Publisher: Springer Science and Business Media LLC
          ISSN (Electronic): 1940-6029
          ISSN (Print): 1064-3745
          Publication date (Electronic): 2021
          Volume: 2239
          Affiliations
          [1 ] Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA.
          [2 ] Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA. kejinhu@uab.edu.
          Article
          DOI: 10.1007/978-1-0716-1084-8_7
          PubMed ID: 33226615
          SO-VID: fd9d6d30-b8b7-4a3e-b602-1c9336086c91
          History

          Keywords: Human induced pluripotent stem cells,Pluripotent reprogramming,Lentiviral vectors,Human iPSC,Fibroblasts,Co-expression of GFP,Chemically defined medium
          Data availability:
          Keywords: Human induced pluripotent stem cells, Pluripotent reprogramming, Lentiviral vectors, Human iPSC, Fibroblasts, Co-expression of GFP, Chemically defined medium

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