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      Adolescent exposure to low-dose THC disrupts energy balance and adipose organ homeostasis in adulthood

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          Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2

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            Analyzing real-time PCR data by the comparative CT method

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              Universal sample preparation method for proteome analysis.

              We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
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                Author and article information

                Contributors
                (View ORCID Profile)
                Journal
                Cell Metabolism
                Cell Metabolism
                Elsevier BV
                15504131
                June 2023
                June 2023
                Article
                10.1016/j.cmet.2023.05.002
                37267956
                fdd6a2b6-dbca-4908-92a0-c9d09341f9dd
                © 2023

                https://www.elsevier.com/tdm/userlicense/1.0/

                http://creativecommons.org/licenses/by-nc-nd/4.0/

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