53
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Cheating evolution: engineering gene drives to manipulate the fate of wild populations.

      1 , 1 , 1
      Nature reviews. Genetics

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Engineered gene drives - the process of stimulating the biased inheritance of specific genes - have the potential to enable the spread of desirable genes throughout wild populations or to suppress harmful species, and may be particularly useful for the control of vector-borne diseases such as malaria. Although several types of selfish genetic elements exist in nature, few have been successfully engineered in the laboratory thus far. With the discovery of RNA-guided CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated 9) nucleases, which can be utilized to create, streamline and improve synthetic gene drives, this is rapidly changing. Here, we discuss the different types of engineered gene drives and their potential applications, as well as current policies regarding the safety and regulation of gene drives for the manipulation of wild populations.

          Related collections

          Most cited references107

          • Record: found
          • Abstract: found
          • Article: not found

          Efficient In Vivo Genome Editing Using RNA-Guided Nucleases

          Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have evolved in bacteria and archaea as a defense mechanism to silence foreign nucleic acids of viruses and plasmids. Recent work has shown that bacterial type II CRISPR systems can be adapted to create guide RNAs (gRNAs) capable of directing site-specific DNA cleavage by the Cas9 nuclease in vitro. Here we show that this system can function in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies comparable to those obtained using ZFNs and TALENs for the same genes. RNA-guided nucleases robustly enabled genome editing at 9 of 11 different sites tested, including two for which TALENs previously failed to induce alterations. These results demonstrate that programmable CRISPR/Cas systems provide a simple, rapid, and highly scalable method for altering genes in vivo, opening the door to using RNA-guided nucleases for genome editing in a wide range of organisms.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.

              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Extraordinary Sex Ratios

              W Hamilton (1967)
                Bookmark

                Author and article information

                Journal
                Nat. Rev. Genet.
                Nature reviews. Genetics
                1471-0064
                1471-0056
                Mar 2016
                : 17
                : 3
                Affiliations
                [1 ] Department of Entomology, University of California, Riverside, Center for Disease Vector Research, Institute for Integrative Genome Biology, University of California, Riverside, California 92521, USA.
                Article
                nrg.2015.34
                10.1038/nrg.2015.34
                26875679
                fe004048-4635-400d-b320-56bca3e28bdb
                History

                Comments

                Comment on this article