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      Dermatopontin is a novel regulator of the CdCl2-induced decrease in claudin-11 expression.

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          Abstract

          Cadmium (Cd) is a ubiquitous environmental heavy metal, which may be harmful to the reproductive functions through injury to the blood-testis barrier (BTB). However, the underlying mechanism of this adverse effect on the BTB remains uncharacterized. A preliminary study revealed that dermatopontin (DPT) expression was significantly increased in Cd chloride (CdCl2)-treated Sertoli cells in vitro, which suggested that an increase in DPT expression is crucial for CdCl2-induced BTB damage. To explore this further, in the present study we initially determined that DPT is expressed in testis Sertoli cells. The treatment of cells with CdCl2 resulted in a significant increase in DPT expression and a parallel decrease in claudin-11 expression, both in vivo and in vitro. To confirm the relationship between DPT and claudin-11, a DPT-silenced 15P-1 Sertoli cell model was established. We determined that DPT silencing could partly reduce the CdCl2-induced decrease in claudin-11 expression. Additionally, western blot analyses demonstrated that the p38 signaling pathway is involved in the effect of CdCl2 on DPT expression. In conclusion, the present study provides the first evidence that DPT may be a novel effector of CdCl2, highlighting the significant role of DPT in the regulation of claudin-11 expression.

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          Author and article information

          Journal
          Toxicol In Vitro
          Toxicology in vitro : an international journal published in association with BIBRA
          Elsevier BV
          1879-3177
          0887-2333
          Sep 2014
          : 28
          : 6
          Affiliations
          [1 ] Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China.
          [2 ] The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
          [3 ] Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China. Electronic address: lg168sn@yahoo.com.
          Article
          S0887-2333(14)00102-7
          10.1016/j.tiv.2014.05.013
          24909373
          fe134dbd-0b4d-4d1a-94c2-b41a30d3978f
          History

          CdCl(2),Claudin-11,Blood-testis barrier,Dermatopontin
          CdCl(2), Claudin-11, Blood-testis barrier, Dermatopontin

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