Performance and Verification of a Real-Time PCR Assay Targeting the gyrA Gene for Prediction of Ciprofloxacin Resistance in Neisseria gonorrhoeae.

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Abstract

In the United States, 19.2% of Neisseria gonorrhoeae isolates are resistant to ciprofloxacin. We evaluated a real-time PCR assay to predict ciprofloxacin susceptibility using residual DNA from the Roche Cobas 4800 CT/NG assay. The results of the assay were 100% concordant with agar dilution susceptibility test results for 100 clinical isolates. Among 76 clinical urine and swab specimens positive for N. gonorrhoeae by the Cobas assay, 71% could be genotyped. The test took 1.5 h to perform, allowing the physician to receive results in time to make informed clinical decisions.

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Journal

Journal ID (iso-abbrev): J. Clin. Microbiol.

Title: Journal of clinical microbiology

Publisher: American Society for Microbiology

ISSN (Electronic): 1098-660X

ISSN (Print): 0095-1137

Publication date (Electronic): Mar 2016

Volume: 54

Issue: 3

Affiliations

[1 ] Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.

[2 ] Neisseria Reference Laboratory, GISP Regional Laboratory, University of Washington Harborview Medical Center, Seattle, Washington, USA.

[3 ] Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA rhumphries@mednet.ucla.edu.

[4 ] Department of Medicine, Division of Infectious Diseases and Department of Epidemiology, David Geffen School of Medicine and Fielding School of Public Health, University of California, Los Angeles, California, USA.

Article

Publisher Item ID: JCM.03032-15

DOI: 10.1128/JCM.03032-15

PMC ID: 4767994

PubMed ID: 26739156

SO-VID: fe9218cc-76cb-42a8-b12e-439836a68a9d

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