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      Diagnostic Electron Microscopy of Viruses With Low-voltage Electron Microscopes.

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          Abstract

          Diagnostic electron microscopy is a useful technique for the identification of viruses associated with human, animal, or plant diseases. The size of virus structures requires a high optical resolution (i.e., about 1 nm), which, for a long time, was only provided by transmission electron microscopes operated at 60 kV and above. During the last decade, low-voltage electron microscopy has been improved and potentially provides an alternative to the use of high-voltage electron microscopy for diagnostic electron microscopy of viruses. Therefore, we have compared the imaging capabilities of three low-voltage electron microscopes, a scanning electron microscope equipped with a scanning transmission detector and two low-voltage transmission electron microscopes, operated at 25 kV, with the imaging capabilities of a high-voltage transmission electron microscope using different viruses in samples prepared by negative staining and ultrathin sectioning. All of the microscopes provided sufficient optical resolution for a recognition of the viruses tested. In ultrathin sections, ultrastructural details of virus genesis could be revealed. Speed of imaging was fast enough to allow rapid screening of diagnostic samples at a reasonable throughput. In summary, the results suggest that low-voltage microscopes are a suitable alternative to high-voltage transmission electron microscopes for diagnostic electron microscopy of viruses.

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          Author and article information

          Journal
          J Histochem Cytochem
          The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
          SAGE Publications
          1551-5044
          0022-1554
          June 2020
          : 68
          : 6
          Affiliations
          [1 ] Advanced Light and Electron Microscopy (ZBS 4), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
          Article
          10.1369/0022155420929438
          32436755
          fef451c0-9910-4341-a03e-62f78cfae2ee
          History

          STEM,infectious disease,negative staining,scanning transmission electron microscopy,thin sectioning,transmission electron microscopy,ultrastructure

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