The concept of employees’ Organizational Citizenship Behavior (OCB)and Organizational Justice (OJ) have been broadly discussed in the current literature in different fields. However, the instrumental role of biographical variables such as gender and age concerning their effect on the employees’ OCB and OJ have been less explored. This study evaluates the effect of these biographical variables on OCB and OJ in the context of Higher Education Institutions (HEIs) of Pakistan. Data were collected from 1019 employees through survey method from the employees working in HEIs of Khyber Pakhtunkhwa, Pakistan. Regression method and ANNOVA were used to analyze the collected data. Findings of the study revealed a significant positive effect of age and gender on the employees’ OCB and OJ in academic settings at HEIs. It is recommended that management and policy makers should take these variables into account while making policies and implementing them in the higher education setting. It is further suggested that results of this study should be replicated in other contexts for getting more reliable and valid results.