Blog
About

36
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Phylogenetic insight into subgenera Idaeobatus and Malachobatus ( Rubus, Rosaceae) inferring from ISH analysis

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Rubus is a large and taxonomically complex genus exhibiting agamospermy, polyploidy and frequent hybridization. The objective of this work was to elucidate rDNA disrtibution pattern and investigate genomic composition of polyploids in 16 Rubus taxa (2 n = 2 x, 3 x, 4 x, 8 x) of two subgenera Idaeobatus and Malachobatus by ISH method.

          Results

          The basic Rubus genome had one 45S rDNA locus, and all the polyploids (except R. setchuenensis) had the expected multiples of this number. Diploid and tetraploid Rubus taxa carried two 5S rDNA, whereas the triploid and octoploid species only had three. The duplicated 45S rDNA sites tended to be conserved, whereas those of 5S rDNA tended to be eliminated after polyploidization. The accession R03-20 was an autotriploid R. parvifolius, while R03-27 and R03-57 were naturally-occurred triploid hybrids between R. parvifolius and R. coreanus. GISH results suggested that R. parvifolius had close relationship with polyploids from Malachobatus.

          Conclusions

          The polyploids from Malachobatus were probable allopolyploid. In addition, Rubus parvifolius might be involved in hybridization, polyploidization and speciation of some Idaeobatus and Malachobatus species.

          Related collections

          Most cited references 70

          • Record: found
          • Abstract: not found
          • Article: not found

          Natural Hybridization as an Evolutionary Process

           M Arnold (1992)
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            On Ö. Winge and a Prayer: The origins of polyploidy

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research.

              Fluorescence in situ hybridization (FISH), which allows direct mapping of DNA sequences on chromosomes, has become the most important technique in plant molecular cytogenetics research. Repetitive DNA sequence can generate unique FISH patterns on individual chromosomes for karyotyping and phylogenetic analysis. FISH on meiotic pachytene chromosomes coupled with digital imaging systems has become an efficient method to develop physical maps in plant species. FISH on extended DNA fibers provides a high-resolution mapping approach to analyze large DNA molecules and to characterize large genomic loci. FISH-based physical mapping provides a valuable complementary approach in genome sequencing and map-based cloning research. We expect that FISH will continue to play an important role in relating DNA sequence information to chromosome biology. FISH coupled with immunoassays will be increasingly used to study features of chromatin at the cytological level that control expression and regulation of genes.
                Bookmark

                Author and article information

                Contributors
                wangyanwxy@163.com
                wangxrtj@163.com
                supnovel@sicau.edu.cn
                zhanglicqsc@163.com
                htang@sicau.edu.cn
                70684075@qq.com
                173698873@qq.com
                Journal
                Mol Cytogenet
                Mol Cytogenet
                Molecular Cytogenetics
                BioMed Central (London )
                1755-8166
                3 February 2015
                3 February 2015
                2015
                : 8
                Affiliations
                [ ]College of Horticulture, Sichuan Agricultural University, Ya’an, 625014 People’s Republic of China
                [ ]Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 611130 People’s Republic of China
                Article
                114
                10.1186/s13039-015-0114-y
                4324050
                © Wang et al.; licensee BioMed Central. 2015

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                Categories
                Research
                Custom metadata
                © The Author(s) 2015

                Genetics

                gish, rubus, rdna-fish, hybrid, allopolyploid

                Comments

                Comment on this article