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      Complementation of bacterial SecE by a chloroplastic homologue.

      1 , , ,
      FEBS letters

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          Abstract

          The SecE protein is an essential component of the SecAYE-translocase, which mediates protein translocation across the cytoplasmic membrane in bacteria. In the thylakoid membranes of chloroplasts, a protein homologous to SecE, chloroplastic (cp) SecE, has been identified. However, the functional role of cpSecE has not been established experimentally. In this report we show that cpSecE in cells depleted for bacterial SecE (i) supports growth, (ii) stabilizes, just like bacterial SecE, the Sec-translocase core component SecY, and (iii) supports Sec-dependent protein translocation. This indicates that cpSecE can functionally replace bacterial SecE in vivo, and strongly suggests that the thylakoid membrane contains a SecAYE-like translocase with functional and structural similarities to the bacterial complex. This study further underscores the evolutionary link between chloroplasts and bacteria.

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          Author and article information

          Journal
          FEBS Lett.
          FEBS letters
          0014-5793
          0014-5793
          Jun 01 2001
          : 498
          : 1
          Affiliations
          [1 ] Department of Biochemistry and Biophysics, Stockholm University, Sweden.
          Article
          S0014-5793(01)02494-2
          11389897
          ff756e55-aee0-4e1e-8031-f8fb21540d73
          History

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