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      A conserved neutralizing epitope on gp41 of human immunodeficiency virus type 1.

      Journal of Biology
      Amino Acid Sequence, Antibodies, Monoclonal, immunology, Base Sequence, Epitopes, HIV Antibodies, HIV Antigens, HIV Envelope Protein gp41, HIV-1, Molecular Sequence Data, Neutralization Tests

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          Abstract

          Vaccination against human immunodeficiency virus type 1 (HIV-1) requires an immunogen which will elicit a protective immunity against viruses that show a high degree of genetic polymorphism. Therefore, the identification of neutralizing epitopes which are shared by many strains would be useful. In previous studies, we established a human monoclonal antibody (2F5) that neutralizes a variety of laboratory strains and clinical isolates of HIV-1. In the present report, we define the amino acid sequence Glu-Leu-Asp-Lys-Trp-Ala (ELDKWA) on the ectodomain of gp41 as the epitope recognized by this antibody. The sequence was found to be conserved in 72% of otherwise highly variable HIV-1 isolates. Escape mutants were not detected in cells infected with HIV-1 isolates MN and RF in the presence of antibody 2F5. Since sequence variability of neutralizing epitopes is considered to be a major obstacle to HIV-1 vaccine development, the conserved B-cell epitope described here is a promising candidate for inclusion in a vaccine against AIDS.

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          Author and article information

          Journal
          7692082
          238102
          10.1128/JVI.67.11.6642-6647.1993

          Chemistry
          Amino Acid Sequence,Antibodies, Monoclonal,immunology,Base Sequence,Epitopes,HIV Antibodies,HIV Antigens,HIV Envelope Protein gp41,HIV-1,Molecular Sequence Data,Neutralization Tests

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