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      Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors.

      1 , , ,
      Cytotechnology
      Springer Nature

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          Abstract

          In recent years, several automated scale-down bioreactor systems have been developed to increase efficiency in cell culture process development. ambr™ is an automated workstation that provides individual monitoring and control of culture dissolved oxygen and pH in single-use, stirred-tank bioreactors at a working volume of 10-15 mL. To evaluate the ambr™ system, we compared the performance of four recombinant Chinese hamster ovary cell lines in a fed-batch process in parallel ambr™, 2-L bench-top bioreactors, and shake flasks. Cultures in ambr™ matched 2-L bioreactors in controlling the environment (temperature, dissolved oxygen, and pH) and in culture performance (growth, viability, glucose, lactate, Na(+), osmolality, titer, and product quality). However, cultures in shake flasks did not show comparable performance to the ambr™ and 2-L bioreactors.

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          Author and article information

          Journal
          Cytotechnology
          Cytotechnology
          Springer Nature
          0920-9069
          0920-9069
          Dec 2012
          : 64
          : 6
          Affiliations
          [1 ] Early Stage Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, CA, 94080, USA, wendyh@gene.com.
          Article
          10.1007/s10616-012-9446-1
          3488368
          22451076
          ffba25cc-1ff8-444a-a56d-0994cba5686c
          History

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