%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Rongsui Gao, Yongfei Hu, Zhencui Li, Jian Sun, Qingjing Wang, Jingxia Lin, Huiyan Ye, Fei Liu, Swaminath Srinivas, Defeng Li, Baoli Zhu, Ya-Hong Liu, Guo-Bao Tian, Youjun Feng
Dissemination and Mechanism for the MCR-1 Colistin Resistance
www.plospathogens.org
www.plospathogens.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 14 0 R/Contents 15 0 R/TrimBox[0 0 612 792]>>
endobj
14 0 obj
[16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R 34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 43 0 R 44 0 R 45 0 R 46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R 54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R]
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref001)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref002)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref002)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref002)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref003)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref003)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref004)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref005)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref006)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref007)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref008)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref006)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref006)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref007)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.g001)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref002)>>
endobj
32 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref007)>>
endobj
33 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref007)>>
endobj
34 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref009)>>
endobj
35 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref010)>>
endobj
36 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref011)>>
endobj
37 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref007)>>
endobj
38 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref012)>>
endobj
39 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref012)>>
endobj
40 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref007)>>
endobj
41 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref013)>>
endobj
42 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref011)>>
endobj
43 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref007)>>
endobj
44 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref011)>>
endobj
45 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref014)>>
endobj
46 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref016)>>
endobj
47 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref017)>>
endobj
48 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref011)>>
endobj
49 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref010)>>
endobj
50 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref010)>>
endobj
51 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref011)>>
endobj
52 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref011)>>
endobj
53 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref018)>>
endobj
54 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref016)>>
endobj
55 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref019)>>
endobj
56 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref021)>>
endobj
57 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref022)>>
endobj
58 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref023)>>
endobj
59 0 obj
<>/Border[0 0 0]/Dest(Rppat.1005957.ref024)>>
endobj
15 0 obj
[60 0 R 61 0 R 62 0 R 63 0 R 64 0 R 65 0 R 66 0 R 67 0 R 68 0 R 69 0 R 70 0 R 71 0 R 72 0 R 73 0 R 74 0 R 75 0 R 76 0 R 77 0 R 78 0 R 79 0 R 80 0 R 81 0 R 82 0 R 83 0 R 84 0 R]
endobj
60 0 obj
<>stream
q
0.955 g
0 w
199.9998 713.9999 m
575.9997 713.9999 l
575.9997 624.0002 l
199.9998 624.0002 l
f*
0.83 0.64 0.02 0 k
393.9024 549.9213 m
398.6646 549.9213 l
h
f*
400.8756 549.9213 m
405.6378 549.9213 l
h
f*
447.4772 510.9165 m
452.1827 510.9165 l
h
f*
422.9291 484.8945 m
427.6913 484.8945 l
h
f*
418.9039 458.9291 m
423.6661 458.9291 l
h
f*
359.4331 445.9465 m
364.1953 445.9465 l
h
f*
330.1228 406.9417 m
334.885 406.9417 l
h
f*
337.0961 406.9417 m
341.8583 406.9417 l
h
f*
541.1339 393.9024 m
545.8961 393.9024 l
h
f*
296.2772 367.937 m
301.0394 367.937 l
h
f*
237.3732 354.8976 m
242.1354 354.8976 l
h
f*
299.0551 354.8976 m
303.8173 354.8976 l
h
f*
410.2866 354.8976 m
415.0488 354.8976 l
h
f*
524.863 341.915 m
529.5685 341.915 l
h
f*
393.6189 302.9102 m
413.178 302.9102 l
h
f*
422.1921 302.9102 m
426.9543 302.9102 l
h
f*
429.222 302.9102 m
433.9276 302.9102 l
h
f*
203.4142 276.9449 m
208.1764 276.9449 l
h
f*
210.3874 276.9449 m
215.1496 276.9449 l
h
f*
217.4173 276.9449 m
226.9417 276.9449 l
h
f*
311.6409 276.9449 m
321.1654 276.9449 l
h
f*
389.9906 276.9449 m
394.7528 276.9449 l
h
f*
497.7638 276.9449 m
507.2882 276.9449 l
h
f*
203.4142 263.9055 m
212.9386 263.9055 l
h
f*
292.3654 250.9228 m
297.0709 250.9228 l
h
f*
299.3386 250.9228 m
308.863 250.9228 l
h
f*
501.5055 237.9402 m
510.9732 237.9402 l
h
f*
540.5669 237.9402 m
545.3291 237.9402 l
h
f*
547.5969 237.9402 m
557.0646 237.9402 l
h
f*
559.3323 237.9402 m
568.8567 237.9402 l
h
f*
200.0126 224.9008 m
209.537 224.9008 l
h
f*
241.5685 224.9008 m
251.0929 224.9008 l
h
f*
303.0803 224.9008 m
312.6047 224.9008 l
h
f*
369.6378 224.9008 m
379.1622 224.9008 l
h
f*
527.1874 211.9181 m
536.7118 211.9181 l
h
f*
422.7024 185.8961 m
432.2268 185.8961 l
h
f*
521.0646 172.9134 m
530.589 172.9134 l
h
f*
358.4693 146.948 m
367.9937 146.948 l
h
f*
510.9165 146.948 m
520.4409 146.948 l
h
f*
522.7087 146.948 m
532.2331 146.948 l
h
f*
537.3921 146.948 m
546.9165 146.948 l
h
f*
559.5591 133.9087 m
569.0268 133.9087 l
h
f*
390.7843 107.9433 m
400.252 107.9433 l
h
f*
219.0047 94.9039 m
228.5291 94.9039 l
h
f*
0 g
1 j
1 J
10 0 0 10 209.9905 696.9826 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(essential)Tj
3.5773 0 Td
(for)Tj
1.3776 0 Td
(its)Tj
1.1395 0 Td
(function.)Tj
3.8494 0 Td
(Phylogenetic)Tj
5.3575 0 Td
(analyses)Tj
3.4752 0 Td
(revealed)Tj
3.515 0 Td
(that)Tj
1.7688 0 Td
(MCR-1)Tj
3.2201 0 Td
(is)Tj
0.8391 0 Td
(highly)Tj
2.7042 0 Td
(homologous)Tj
-30.8237 -1.2982 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(PEA)Tj
2.0353 0 Td
(lipid)Tj
2.0466 0 Td
(A)Tj
0.9014 0 Td
(transferase)Tj
4.5354 0 Td
(in)Tj
/F8 1 Tf
1.0375 0 Td
[()23()6()2( )18(\n)14()4()6()1(\n)4()4()12(\r)-4()]TJ
/F7 1 Tf
(,)Tj
5.5955 0 Td
(a)Tj
0.652 0 Td
(known)Tj
3.0047 0 Td
(producer)Tj
3.8891 0 Td
(of)Tj
1.0148 0 Td
(polymyxins.)Tj
5.0853 0 Td
(The)Tj
1.7858 0 Td
(fact)Tj
-34.0778 -1.2983 Td
(that)Tj
1.7801 0 Td
(the)Tj
1.4683 0 Td
(plasmid-borne)Tj
6.1002 0 Td
(MCR-1)Tj
3.2258 0 Td
(is)Tj
0.8447 0 Td
(placed)Tj
2.7949 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.652 0 Td
(subclade)Tj
3.668 0 Td
(neighboring)Tj
5.1193 0 Td
(the)Tj
1.4683 0 Td
(chromosome-)Tj
-28.1591 -1.3039 Td
(encoded)Tj
3.5999 0 Td
(colistin-resistant)Tj
/F8 1 Tf
6.8542 0 Td
[()28()2(\n)-4()10()-3()2()-9(\n)4()]TJ
/F7 1 Tf
3.7417 0 Td
(LptA)Tj
2.2563 0 Td
(potentially)Tj
4.4617 0 Td
(implies)Tj
3.1238 0 Td
(parallel)Tj
3.1351 0 Td
(evolutionary)Tj
5.2611 0 Td
(paths)Tj
-32.4338 -1.2983 Td
(for)Tj
1.3889 0 Td
(the)Tj
1.4684 0 Td
(two)Tj
1.7064 0 Td
(genes.)Tj
2.6702 0 Td
(Our)Tj
1.8652 0 Td
(results)Tj
2.8063 0 Td
(reveal)Tj
2.5738 0 Td
(mechanistic)Tj
5.0173 0 Td
(insights)Tj
3.3165 0 Td
(into)Tj
1.8369 0 Td
(the)Tj
1.474 0 Td
(MCR-1-mediated)Tj
7.2963 0 Td
(colis-)Tj
-33.4202 -1.2982 Td
(tin)Tj
1.3322 0 Td
(resistance.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 580.9889 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Introduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 563.9811 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(polymyxins)Tj
4.8642 0 Td
(\(polymyxin)Tj
4.8416 0 Td
(E)Tj
0.788 0 Td
(\(colistin\))Tj
3.8154 0 Td
(and)Tj
1.7348 0 Td
(polymyxin)Tj
4.4957 0 Td
(B\))Tj
1.1452 0 Td
(are)Tj
1.4457 0 Td
(a)Tj
0.6519 0 Td
(family)Tj
2.7326 0 Td
(of)Tj
1.0148 0 Td
(cationic)Tj
3.3902 0 Td
(polypeptide)Tj
-32.7002 -1.2982 Td
(antibiotics)Tj
4.3993 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.6519 0 Td
(lipophilic)Tj
4.0082 0 Td
(fatty)Tj
1.9956 0 Td
(acyl)Tj
1.7745 0 Td
(side)Tj
1.8028 0 Td
(chain)Tj
2.4207 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(].)Tj
2.3018 0 Td
(The)Tj
1.7801 0 Td
(initial)Tj
2.5512 0 Td
(binding)Tj
3.3505 0 Td
(of)Tj
1.0148 0 Td
(polymyxins)Tj
4.8586 0 Td
(bacte-)Tj
-34.9056 -1.2983 Td
(rial)Tj
1.5363 0 Td
(surface)Tj
3.0501 0 Td
(mainly)Tj
2.9877 0 Td
(depends)Tj
3.5489 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.474 0 Td
(electrostatic)Tj
4.9946 0 Td
(interaction)Tj
4.6091 0 Td
(between)Tj
3.5263 0 Td
(the)Tj
1.4684 0 Td
(positively-charged)Tj
-28.471 -1.3039 Td
(polymyxin)Tj
4.5013 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.4683 0 Td
(negatively-charged)Tj
7.7612 0 Td
(phosphate)Tj
4.3313 0 Td
(group)Tj
2.6079 0 Td
(of)Tj
1.0148 0 Td
(lipid)Tj
2.0466 0 Td
(A)Tj
0.9071 0 Td
(on)Tj
1.2755 0 Td
(lipopolysaccharide)Tj
7.6762 0 Td
(\(LPS\))Tj
-35.3194 -1.2983 Td
(localized)Tj
3.7076 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4684 0 Td
(outer)Tj
2.347 0 Td
(leaflet)Tj
2.5795 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(bacterial)Tj
3.6283 0 Td
(outer)Tj
2.3471 0 Td
(membrane)Tj
4.5694 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(].)Tj
1.5987 0 Td
(Following)Tj
4.218 0 Td
(its)Tj
1.1451 0 Td
(diffusion)Tj
3.8041 0 Td
(from)Tj
-35.172 -1.2982 Td
(the)Tj
1.4683 0 Td
(outer)Tj
2.3471 0 Td
(membrane)Tj
4.5694 0 Td
(across)Tj
2.6759 0 Td
(the)Tj
1.4683 0 Td
(periplasm,)Tj
4.405 0 Td
(polymyxin)Tj
4.5014 0 Td
(intercalates)Tj
4.7452 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
1.4683 0 Td
(inner)Tj
2.3811 0 Td
(membrane)Tj
-31.8725 -1.3039 Td
(and)Tj
1.7291 0 Td
(forms)Tj
2.5681 0 Td
(pores,)Tj
2.6249 0 Td
(which)Tj
2.6476 0 Td
(in)Tj
1.0374 0 Td
(turn)Tj
1.9673 0 Td
(results)Tj
2.8062 0 Td
(in)Tj
1.0375 0 Td
(bacterial)Tj
3.6227 0 Td
(lysis)Tj
1.9105 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(].)Tj
1.6044 0 Td
(Although)Tj
4.0025 0 Td
(they)Tj
1.9219 0 Td
(belong)Tj
2.9196 0 Td
(to)Tj
1.0262 0 Td
(an)Tj
1.2019 0 Td
(old)Tj
-34.6278 -1.2983 Td
(generation)Tj
4.507 0 Td
(of)Tj
1.0148 0 Td
(antibiotics,)Tj
4.6204 0 Td
(polymyxins)Tj
4.8643 0 Td
(represent)Tj
3.9571 0 Td
(the)Tj
1.4683 0 Td
(last)Tj
1.5591 0 Td
(line)Tj
1.7064 0 Td
(of)Tj
1.0148 0 Td
(defense)Tj
3.2202 0 Td
(against)Tj
3.0274 0 Td
(lethal)Tj
2.3924 0 Td
(infections)Tj
-33.3522 -1.2983 Td
(by)Tj
1.1792 0 Td
(gram-negative)Tj
5.9754 0 Td
(pathogens)Tj
4.3029 0 Td
(with)Tj
1.9956 0 Td
(pan-drug)Tj
3.9628 0 Td
(resistance)Tj
4.1329 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(].)Tj
1.6044 0 Td
(Unfortunately,)Tj
6.1285 0 Td
(certain)Tj
2.982 0 Td
(species)Tj
2.999 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-36.2775 -1.2982 Td
(Enterobacteriaceae)Tj
7.8008 0 Td
(like)Tj
/F8 1 Tf
1.6498 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0885 0 Td
[()11( )6()2(\r)12()6()11( )18(\n)4()6()]TJ
/F7 1 Tf
5.0626 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(])Tj
1.372 0 Td
(have)Tj
2.0636 0 Td
(been)Tj
2.1203 0 Td
(recently)Tj
3.4129 0 Td
(showing)Tj
3.5773 0 Td
(an)Tj
1.2019 0 Td
(appreciable)Tj
4.7848 0 Td
(resis-)Tj
-34.1345 -1.304 Td
(tance)Tj
2.347 0 Td
(to)Tj
1.0262 0 Td
(colistin.)Tj
3.3618 0 Td
(Indeed,)Tj
3.2372 0 Td
(colistin)Tj
3.1407 0 Td
(resistance)Tj
4.1273 0 Td
(\(i.e.,)Tj
1.9275 0 Td
(inefficient)Tj
4.2633 0 Td
(binding)Tj
3.3505 0 Td
(of)Tj
1.0148 0 Td
(polymyxins)Tj
4.8642 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(lipid)Tj
-35.155 -1.2982 Td
(A)Tj
0.9014 0 Td
(moiety)Tj
2.9933 0 Td
(of)Tj
1.0148 0 Td
(lipopolysaccharide\))Tj
8.0164 0 Td
(is)Tj
0.8447 0 Td
(mainly)Tj
2.9933 0 Td
(due)Tj
1.6951 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(4'-phosphoethanolamin)Tj
9.5641 0 Td
(e)Tj
0.6406 0 Td
(\(PEA\))Tj
2.7156 0 Td
(modifi-)Tj
-33.8738 -1.2983 Td
(cation)Tj
2.6985 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(lipid)Tj
2.0467 0 Td
(A)Tj
0.9014 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
(LPS)Tj
1.7971 0 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(].)Tj
2.3018 0 Td
(This)Tj
1.9842 0 Td
(type)Tj
1.9162 0 Td
(of)Tj
1.0148 0 Td
(chemical)Tj
3.7871 0 Td
(modification)Tj
5.3744 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(bacterial)Tj
3.6227 0 Td
(lipid)Tj
-35.4158 -1.3039 Td
(A)Tj
0.9014 0 Td
(can)Tj
1.627 0 Td
(be)Tj
1.1509 0 Td
(attributed)Tj
4.1726 0 Td
(to)Tj
1.0261 0 Td
(either)Tj
2.5342 0 Td
(the)Tj
1.4683 0 Td
(chromosome-encoded)Tj
9.2182 0 Td
(machinery)Tj
4.4901 0 Td
(in)Tj
/F8 1 Tf
1.0374 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0829 0 Td
[()11( )6()2(\r)12()6()11( )18(\n)4()6()]TJ
/F7 1 Tf
5.0626 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(])Tj
1.3776 0 Td
(or)Tj
1.0942 0 Td
(the)Tj
-36.2435 -1.2983 Td
(plasmid-transferred)Tj
8.158 0 Td
(mobilized)Tj
4.2009 0 Td
(colistin)Tj
3.1408 0 Td
(resistance)Tj
4.1272 0 Td
(\(MCR-1\))Tj
3.9061 0 Td
(gene)Tj
2.0806 0 Td
(in)Tj
1.0375 0 Td
(certain)Tj
2.982 0 Td
(species)Tj
2.9991 0 Td
(of)Tj
1.0148 0 Td
(Entero-)Tj
-33.647 -1.2982 Td
(bacteriaceae)Tj
5.0796 0 Td
(like)Tj
/F8 1 Tf
1.6554 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9921 0 Td
[()7()10()12(\n)]TJ
/F7 1 Tf
1.5591 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
1.6044 0 Td
(For)Tj
1.6271 0 Td
(the)Tj
1.4683 0 Td
(former,)Tj
3.2258 0 Td
(two)Tj
1.7065 0 Td
(sets)Tj
1.6667 0 Td
(of)Tj
1.0148 0 Td
(bacterial)Tj
3.6227 0 Td
(two-component)Tj
6.6557 0 Td
(systems)Tj
-31.8782 -1.3039 Td
(\()Tj
/F8 1 Tf
[()11()18()]TJ
/F7 1 Tf
3.3958 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(])Tj
1.3777 0 Td
(plus)Tj
/F8 1 Tf
1.8708 0 Td
[()10()6()]TJ
/F7 1 Tf
2.9197 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(]\))Tj
1.7178 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(regulator)Tj
/F8 1 Tf
3.8665 0 Td
[()13()-8()]TJ
/F7 1 Tf
2.3414 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(])Tj
1.3776 0 Td
(are)Tj
1.4457 0 Td
(implicated,)Tj
4.6714 0 Td
(in)Tj
1.0375 0 Td
(which)Tj
2.6475 0 Td
(the)Tj
1.4684 0 Td
(lipid)Tj
2.0466 0 Td
(A)Tj
0.9071 0 Td
(of)Tj
-36.2889 -1.2983 Td
(LPS)Tj
1.7914 0 Td
(is)Tj
0.8448 0 Td
(chemically)Tj
4.4843 0 Td
(modified)Tj
3.8495 0 Td
(and)Tj
1.7291 0 Td
(thereafter)Tj
4.0762 0 Td
(exhibits)Tj
3.3335 0 Td
(reduced)Tj
3.4469 0 Td
(affinity)Tj
3.0614 0 Td
(to)Tj
1.0261 0 Td
(polymyxin)Tj
4.5014 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
1.5988 0 Td
(The)Tj
1.7801 0 Td
(latter)Tj
-35.5235 -1.2983 Td
(represents)Tj
4.3199 0 Td
(an)Tj
1.2019 0 Td
(unique)Tj
3.0217 0 Td
(mechanism)Tj
4.8416 0 Td
(for)Tj
1.3889 0 Td
(bacterial)Tj
3.6227 0 Td
(colistin)Tj
3.1408 0 Td
(resistance)Tj
4.1328 0 Td
(in)Tj
1.0318 0 Td
(that)Tj
1.7802 0 Td
(the)Tj
/F8 1 Tf
1.4683 0 Td
[()6()1()19()]TJ
/F7 1 Tf
2.5285 0 Td
(gene)Tj
2.0806 0 Td
(prod-)Tj
-34.5597 -1.2982 Td
(uct,)Tj
1.6951 0 Td
(annotated)Tj
4.2406 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6519 0 Td
(member)Tj
3.583 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
0.6576 0 Td
(family)Tj
2.7269 0 Td
(of)Tj
1.0148 0 Td
(phosphoethanolamine)Tj
9.1615 0 Td
(transferases,)Tj
5.1251 0 Td
(catalyzes)Tj
3.7076 0 Td
(the)Tj
-34.5937 -1.304 Td
(modification)Tj
5.3744 0 Td
(of)Tj
1.0148 0 Td
(lipid)Tj
2.0466 0 Td
(A)Tj
0.9014 0 Td
(moiety)Tj
2.9934 0 Td
(on)Tj
1.2756 0 Td
(bacterial)Tj
3.6226 0 Td
(LPS)Tj
1.7915 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1)Tj
0 g
(\))Tj
1.0375 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
2.3017 0 Td
(To)Tj
1.338 0 Td
(the)Tj
1.4683 0 Td
(best)Tj
1.8085 0 Td
(of)Tj
1.0148 0 Td
(our)Tj
1.6271 0 Td
(knowledge,)Tj
4.7678 0 Td
(the)Tj
-36.2038 -1.2982 Td
(natural)Tj
3.0784 0 Td
(occurrence)Tj
4.6658 0 Td
(of)Tj
1.0147 0 Td
(the)Tj
/F8 1 Tf
1.4684 0 Td
[()6()1()]TJ
/F7 1 Tf
(-)Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
2.5455 0 Td
(gene)Tj
2.0806 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.126 0 Td
(traced)Tj
2.6929 0 Td
(to)Tj
1.0261 0 Td
(no)Tj
1.2756 0 Td
(less)Tj
1.6158 0 Td
(than)Tj
2.0239 0 Td
(five)Tj
1.6554 0 Td
(species:)Tj
/F8 1 Tf
3.2201 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9978 0 Td
[()7()10()12(\n)]TJ
/F7 1 Tf
-33.029 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(],)Tj
/F8 1 Tf
3.4752 0 Td
[()-6()11()12()6()11( )6()15()4()4()]TJ
4.5184 0 Td
[()2( )22()11()2()-9(\n)4()]TJ
/F7 1 Tf
2.829 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(],)Tj
/F8 1 Tf
2.0806 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0885 0 Td
[()11( )6()2(\r)12()6()11( )18(\n)4()]TJ
/F7 1 Tf
4.6658 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(],)Tj
/F8 1 Tf
1.5987 0 Td
[()16( )22()11()2()20()13()4()6()-4()11()2()]TJ
5.1987 0 Td
[()6()2()20()2()2()2( )6()8()]TJ
/F7 1 Tf
3.9798 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(])Tj
1.8539 0 Td
(and)Tj
/F8 1 Tf
1.7291 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9921 0 Td
[()13()4()4()6()7()6()]TJ
/F7 1 Tf
-34.0098 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(])Tj
1.8481 0 Td
(\(of)Tj
1.355 0 Td
(note,)Tj
2.2223 0 Td
(it)Tj
0.7881 0 Td
(was)Tj
1.7007 0 Td
(also)Tj
1.7632 0 Td
(experimentally)Tj
6.1625 0 Td
(spread/transmitted)Tj
7.8292 0 Td
(from)Tj
/F8 1 Tf
2.211 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9921 0 Td
[()7()10()12(\n)]TJ
/F7 1 Tf
1.5591 0 Td
(to)Tj
/F8 1 Tf
1.0261 0 Td
[()18()-3()2(\r)4()4()11()6()11( )6()]TJ
5.5672 0 Td
[()6()2()-9(\r)]TJ
-35.0246 -1.2983 Td
[()-8(\n)12( )6()7()-3()]TJ
/F7 1 Tf
2.7099 0 Td
(by)Tj
1.1792 0 Td
(conjugation)Tj
5.0059 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(].\).)Tj
3.3392 0 Td
(Also,)Tj
2.245 0 Td
(the)Tj
1.4684 0 Td
(range)Tj
2.4661 0 Td
(of)Tj
1.0148 0 Td
(host)Tj
1.9105 0 Td
(reservoirs)Tj
4.1329 0 Td
(with)Tj
1.9956 0 Td
(potential)Tj
3.7644 0 Td
(to)Tj
1.0204 0 Td
(carry)Tj
2.2791 0 Td
(the)Tj
/F8 1 Tf
-34.5314 -1.2982 Td
[()6()1()19()]TJ
/F7 1 Tf
(-harbouring)Tj
7.3983 0 Td
(enterobacteria)Tj
5.9527 0 Td
(extends)Tj
3.2712 0 Td
(from)Tj
2.2053 0 Td
(poultry/livestock)Tj
6.9506 0 Td
(\(chickens)Tj
4.0308 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(],)Tj
2.0749 0 Td
(pigs)Tj
1.8312 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
-33.715 -1.304 Td
(16)Tj
0 g
(],)Tj
1.7347 0 Td
(dogs)Tj
2.0807 0 Td
([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(],)Tj
2.0749 0 Td
(and)Tj
1.7348 0 Td
(cattle)Tj
2.3414 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(]\))Tj
2.194 0 Td
(to)Tj
1.0205 0 Td
(humans)Tj
3.4412 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(],)Tj
2.075 0 Td
(and)Tj
1.7347 0 Td
(published)Tj
4.1216 0 Td
(data)Tj
1.9162 0 Td
(from)Tj
2.2053 0 Td
(January-April)Tj
5.7543 0 Td
(2016)Tj
-34.4293 -1.2982 Td
(suggests)Tj
3.4809 0 Td
(that)Tj
1.7801 0 Td
(the)Tj
/F8 1 Tf
1.4684 0 Td
[()6()1()]TJ
/F7 1 Tf
(-)Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
2.5455 0 Td
(gene)Tj
2.0806 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.126 0 Td
(disseminated)Tj
5.4765 0 Td
(into)Tj
1.8425 0 Td
(no)Tj
1.2756 0 Td
(less)Tj
1.61 0 Td
(than)Tj
2.0296 0 Td
(18)Tj
1.1679 0 Td
(countries)Tj
3.9515 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(].)Tj
2.0749 0 Td
(To)Tj
1.338 0 Td
(a)Tj
-35.79 -1.2983 Td
(certain)Tj
2.9876 0 Td
(degree,)Tj
3.0785 0 Td
(the)Tj
1.4683 0 Td
(global)Tj
2.6249 0 Td
(spread)Tj
2.8573 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F8 1 Tf
1.4683 0 Td
[()6()1()]TJ
/F7 1 Tf
(-)Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
2.5455 0 Td
(gene)Tj
2.0806 0 Td
(might)Tj
2.5965 0 Td
(be)Tj
1.1509 0 Td
(related)Tj
2.948 0 Td
(to)Tj
1.0261 0 Td
(a)Tj
0.652 0 Td
(food-chain)Tj
4.5978 0 Td
(based)Tj
2.4774 0 Td
(dis-)Tj
-35.5745 -1.3039 Td
(semination)Tj
4.6941 0 Td
(pathway,)Tj
3.7927 0 Td
(which)Tj
2.6532 0 Td
(was)Tj
1.7008 0 Td
(shown)Tj
2.846 0 Td
(by)Tj
1.1792 0 Td
(Zhu's)Tj
2.4548 0 Td
(group)Tj
2.6078 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
2.075 0 Td
(Thus,)Tj
2.4774 0 Td
(they)Tj
1.9219 0 Td
(observed)Tj
3.7928 0 Td
(the)Tj
1.474 0 Td
(paralleled)Tj
-33.6697 -1.2983 Td
(existence)Tj
3.855 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()6()1()]TJ
/F7 1 Tf
(-)Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
2.5455 0 Td
(in)Tj
1.0318 0 Td
(meat/food)Tj
4.354 0 Td
(samples)Tj
3.3846 0 Td
(and)Tj
1.7291 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(healthy)Tj
3.1351 0 Td
(human)Tj
3.0784 0 Td
(microbiome)Tj
5.1307 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
2.0749 0 Td
(Worry-)Tj
-33.8397 -1.2982 Td
(ingly,)Tj
2.4264 0 Td
(the)Tj
1.4683 0 Td
(MCR-1)Tj
3.2258 0 Td
(colistin)Tj
3.1351 0 Td
(resistance)Tj
4.1329 0 Td
(gene)Tj
2.0806 0 Td
(was)Tj
1.7008 0 Td
(strikingly)Tj
3.9968 0 Td
(shown)Tj
2.846 0 Td
(to)Tj
1.0261 0 Td
(coexist)Tj
2.9651 0 Td
(with)Tj
1.9955 0 Td
(other)Tj
2.3528 0 Td
(multiple-)Tj
-33.3522 -1.2983 Td
(drug)Tj
2.1089 0 Td
(resistance)Tj
4.1329 0 Td
(genes)Tj
2.4434 0 Td
(\(i.e,)Tj
1.7008 0 Td
(carbapenem)Tj
5.1194 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(])Tj
1.8538 0 Td
(and)Tj
1.7291 0 Td
(extended-spectrum)Tj
/F13 1 Tf
7.954 0 Td
()Tj
/F7 1 Tf
(-lactam)Tj
3.7077 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(]\),)Tj
-30.75 -1.3039 Td
(highlighting)Tj
5.0909 0 Td
(the)Tj
1.4684 0 Td
(possibility)Tj
4.2746 0 Td
(that)Tj
1.7801 0 Td
(micro-organisms)Tj
7.0923 0 Td
(with)Tj
1.9956 0 Td
(pan-drug)Tj
3.9628 0 Td
(resistances)Tj
4.4957 0 Td
(are)Tj
1.4457 0 Td
(emerging)Tj
4.0081 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(].)Tj
-35.6142 -1.2983 Td
(For)Tj
1.627 0 Td
(instance,)Tj
3.7474 0 Td
(a)Tj
0.652 0 Td
(variant)Tj
3.0274 0 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4741 0 Td
(notorious)Tj
4.1158 0 Td
(NDM-1)Tj
3.4073 0 Td
(was)Tj
1.7007 0 Td
(detected)Tj
3.566 0 Td
(to)Tj
1.0261 0 Td
(coexist)Tj
2.9651 0 Td
(with)Tj
1.9955 0 Td
(MCR-1)Tj
3.2258 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
-34.5824 -1.2982 Td
(Enterobacteriaceae)Tj
7.8008 0 Td
(\(NDM-5)Tj
3.7531 0 Td
(in)Tj
/F8 1 Tf
1.0318 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0885 0 Td
[()11( )6()2(\r)12()6()11( )18(\n)4()6()]TJ
/F7 1 Tf
5.0626 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(])Tj
1.8482 0 Td
(and)Tj
1.7291 0 Td
(NDM-9)Tj
3.4129 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.652 0 Td
(chicken)Tj
3.3335 0 Td
(meat)Tj
2.194 0 Td
(isolate)Tj
2.7553 0 Td
(of)Tj
/F8 1 Tf
1.0147 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
-36.714 -1.304 Td
[()7()10()12(\n)]TJ
/F7 1 Tf
1.559 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(]\).)Tj
2.4151 0 Td
(So)Tj
1.2019 0 Td
(far,)Tj
1.542 0 Td
(most)Tj
2.2054 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(studies)Tj
2.9877 0 Td
(in)Tj
1.0318 0 Td
(this)Tj
1.6781 0 Td
(field)Tj
1.9729 0 Td
(focused)Tj
3.2768 0 Td
(on)Tj
1.2756 0 Td
(epidemiological)Tj
6.5593 0 Td
(investigations,)Tj
-30.1887 -1.2982 Td
(which)Tj
2.6475 0 Td
(is)Tj
0.8504 0 Td
(in)Tj
1.0375 0 Td
(part)Tj
1.8368 0 Td
(due)Tj
1.7008 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(relatively)Tj
3.8381 0 Td
(limited)Tj
3.0728 0 Td
(availability)Tj
4.5297 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(genomic)Tj
3.6737 0 Td
(information.)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 144.5669 23.6976 re
W* n
q
144.5102 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 419.8677 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Mechanism)Tj
5.2512 0 Td
(of)Tj
1.0417 0 Td
(the)Tj
1.5874 0 Td
(MCR-1)Tj
3.3378 0 Td
(Colistin)Tj
3.43 0 Td
(Resistanc)Tj
4.3158 0 Td
(e)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Pathogens)Tj
4.9252 0 Td
(|)Tj
0.4748 0 Td
(DOI:10.137)Tj
5.0386 0 Td
(1/journ)Tj
2.9906 0 Td
(al.ppat.100595)Tj
6.4985 0 Td
(7)Tj
1.5449 0 Td
(Novemb)Tj
3.6638 0 Td
(er)Tj
1.0913 0 Td
(28,)Tj
1.5875 0 Td
(2016)Tj
34.4695 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(19)Tj
/F11 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(Award.)Tj
2.8134 0 Td
(YH)Tj
1.3323 0 Td
(is)Tj
0.8362 0 Td
(a)Tj
0.6378 0 Td
(member)Tj
3.352 0 Td
(of)Tj
0.9496 0 Td
(the)Tj
1.3677 0 Td
(Youth)Tj
2.4237 0 Td
(Innovati)Tj
2.9622 0 Td
(on)Tj
-16.6749 -1.3748 Td
(Promotion)Tj
4.1457 0 Td
(Association)Tj
4.4929 0 Td
(of)Tj
0.9496 0 Td
(Chinese)Tj
3.1536 0 Td
(Academ)Tj
3.0118 0 Td
(y)Tj
0.6449 0 Td
(of)Tj
-16.3985 -1.3749 Td
(Sciences\(201)Tj
5.0244 0 Td
(5069\).)Tj
2.6504 0 Td
(The)Tj
1.5874 0 Td
(funders)Tj
3.0473 0 Td
(had)Tj
1.5874 0 Td
(no)Tj
1.1622 0 Td
(role)Tj
1.6158 0 Td
(in)Tj
-16.6749 -1.3748 Td
(study)Tj
2.2677 0 Td
(design,)Tj
2.8984 0 Td
(data)Tj
1.793 0 Td
(collection)Tj
3.7346 0 Td
(and)Tj
1.5874 0 Td
(analysis,)Tj
3.4158 0 Td
(decision)Tj
-15.6969 -1.3748 Td
(to)Tj
0.9496 0 Td
(publish,)Tj
3.1464 0 Td
(or)Tj
0.9993 0 Td
(preparation)Tj
4.4433 0 Td
(of)Tj
0.9425 0 Td
(the)Tj
1.3748 0 Td
(manuscript.)Tj
/F10 1 Tf
-11.8559 -2.126 Td
(Competing)Tj
4.3795 0 Td
(Interests)Tj
3.3307 0 Td
(:)Tj
/F11 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3748 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
61 0 obj
<>stream
endstream
endobj
62 0 obj
<>stream
endstream
endobj
63 0 obj
<>stream
endstream
endobj
64 0 obj
<>stream
endstream
endobj
65 0 obj
<>stream
endstream
endobj
66 0 obj
<>stream
endstream
endobj
67 0 obj
<>stream
endstream
endobj
68 0 obj
<>stream
endstream
endobj
69 0 obj
<>stream
endstream
endobj
70 0 obj
<>stream
endstream
endobj
71 0 obj
<>stream
endstream
endobj
72 0 obj
<>stream
endstream
endobj
73 0 obj
<>stream
endstream
endobj
74 0 obj
<>stream
endstream
endobj
75 0 obj
<>stream
endstream
endobj
76 0 obj
<>stream
endstream
endobj
77 0 obj
<>stream
endstream
endobj
78 0 obj
<>stream
endstream
endobj
79 0 obj
<>stream
endstream
endobj
80 0 obj
<>stream
endstream
endobj
81 0 obj
<>stream
endstream
endobj
82 0 obj
<>stream
endstream
endobj
83 0 obj
<>stream
endstream
endobj
84 0 obj
<>stream
BT
10.3394 75.0903 Td
(exist.)Tj
ET
Q
endstream
endobj
85 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 89 0 R/Contents 90 0 R/TrimBox[0 0 612 792]>>
endobj
88 0 obj
<>stream
JFIF C
$, !$4.763.22:ASF:=N>22HbINVX]^]8EfmeZlS[]Y C**Y;2;YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY "
} !1AQa"q2#BR$3br
%&'()*456789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz
w !1AQaq"2B #3Rbr
$4%&'()*56789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz ? (+jmWr7Z s uMZq,| =(uttGssC++iA6nٖͶ # ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (-!2̐3T ZXQ ngH`FW;UW4O?&p>ҭx"Bla2i1F:!GY20e=9
AGF{Ry?+oC^m