This study aims to identify the population of the airborne bacteria in a hospital and its antibiotic resistant. The airborne bacteria were collected using SKC Biostage Single Stage at three locations in a tertiary hospital in Kuala Lumpur. Temperature, relative humidity and air velocity of the sampling locations were determined using TSI VelociCalc. The screening of antibiotic resistance was conducted using disk diffusion of Kirby-Bauer method. The results show Micrococcus spp., Staphylococcus aureus, α and ȣ- Streptococcus spp., Bacillus spp., Clostridium spp. are the group of bacteria found at the sampling locations. The antibiotic resistance screening shows a wide percentage of resistance among the identified bacteria, with ampicillin as the highest percentage for the bacterial resistance.