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Development of LAMP-HRM for sensitive and specific detection of Phytophthora infestans

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HRM, pathogen variability, pathogen detection, LAMP, tomato late blight, Phytophthora infestans

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      We developed the LAMP-HRM assay for detection of P. infestans in a RealTime PCR machine. Specific pathogen detection was achieved in the 18th minute of the reaction, which, in comparison with other diagnostic methods, shortens the analysis time. For instance, first late blight symptoms after inoculation of tomato plants with P. infestans are manifested only 4-5 dpi. We applied the downstream HRM analyses to estimate the variablility of 96 pathogen isolates, collected from symptomatic tomatoes all over Poland, in the years 2009-2012.

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