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Development of LAMP-HRM for sensitive and specific detection of Phytophthora infestans

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This work has been published open access under Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com.

HRM, pathogen variability, pathogen detection, LAMP, tomato late blight, Phytophthora infestans

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      Abstract

      We developed the LAMP-HRM assay for detection of P. infestans in a RealTime PCR machine. Specific pathogen detection was achieved in the 18th minute of the reaction, which, in comparison with other diagnostic methods, shortens the analysis time. For instance, first late blight symptoms after inoculation of tomato plants with P. infestans are manifested only 4-5 dpi. We applied the downstream HRM analyses to estimate the variablility of 96 pathogen isolates, collected from symptomatic tomatoes all over Poland, in the years 2009-2012.

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      10.14293/P2199-8442.1.SOP-AG.PZXDWN.v1

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