We development method for identification of abamectin and ivermectin in the presence of gentamicin sulfate (interfered substances) using liquid chromatography coupled to an ultra be efficiency photodiodes arrangement (UPLC-DAD). The results of the simultaneous identification of compounds different classes in a single analysis with running time of 20 min, being that the sample was fortified with gentamicin sulfate interference using as to verify the effective of the method. The methodology developed using UPLC-DAD is useful identify a mixture of macrolides and is considered the first step to quality control of veterinary drugs, which aims to promote safety and guarantee the minimization of waste generated in foodstuffs of animal origin. The procedures recommended by the National plan for the Control of residues and contaminants of Ministry of agriculture, livestock and food supply of Brazil (PNCRC/MAPA). Therefore, this method used as a standard inspection method to meet the requirement of the MRL set by the regulation authorities and can extended to other macrolide antibiotics.