Urolithiasis or kidney stone disease is a urologic ailment that has a high prevalence rate worldwide and medicinalplants have been widely used for alternative therapy. A wide range of medicinal plants have been used conservativelyfor urolithiasis due to their fewer side effects and because they contain copious phytochemicals that showadvantageous effects in urolithiasis. Cuminum cyminum traditionally used in the treatment of urolithiasis.Hyperoxaluria and hypercalciuria are among the major risk factors in pathogenesis of urinary stone formation.Evaluation of various medicinal plants are done mainly against calcium oxalate. In this present study, theantiurolithiatic potential in Cuminum cyminum was investigated through invitro assay. The work was performed byusing invitro Anti-urolithiatic model for calculating percentage in comparison with standard drug cystone. The antiurolithiatic properties were evaluated by titrimetric and turbidity assay. The result shows that for C. cyminum folds(55%) and for standard drug cystone (82%) for titrimetric assay. For turbidity assay. C. cyminum has significantproperties (47.9±19%). The findings of the nucleation assay indicate that phytoconstituents inhibited thecrystallization of CaOx in solution. The size and the number of calcium oxalate crystals decreased with increasingconcentration upto 400µg of the phytoconstituents. The study concludes that the seed extracts of Cuminum cyminumhave inhibitory effect on calcium oxalate for crystal nucleation. It also showed great efficacy in the dissolution ofcalcium oxalate crystals. Thus, this extract may be valuable resources for treatment of urolithiasis.