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      Hand in Hand approach by culture and PCR a plausible solution for effective management of risks associated with Legionella in water samples

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      ScienceOpen

      Real time PCR; Legionella, Risk assessment; Culture; Quantification

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          Abstract

          Legionella in water distribution systems appear to be an unescapable threat to public health. The intensity of Legionellosis may vary from mild respiratory illness to potentially deadly pneumonia. Global incidences of Legionnaires disease outbreak has been raising over the years. Thus reliable analytical procedures play a crucial role in the evaluation of risk assessment and control of Legionella. Analytical methods involving traditional culture method and PCR based rapid methods are currently being used in water quality monitoring. While each method has its own advantages and limitations of underestimating and overestimating the occurrence of Legionella, we propose simultaneous application of culture and real time PCR for effective monitoring of Legionella in water distribution systems.

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          Author and article information

          Journal
          ScienceOpen
          11 March 2019
          Affiliations
          [1 ] Central Testing Laboratories, Quality and Conformity Council, Abu Dhabi
          Article
          10.14293/S2199-1006.1.SOR-.PP1JELQ.v1

          This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

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