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      Phosphorylation Dependent Sequestration Of Protein Phosphatase-1 I (PP-1 I) By Protein 14-3-3

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            Abstract

            PP-1 is involved in the control of the meiotic and mitotic cell divisions at the G2 to M transition by dephosphorylating phospho-serine 217 (phospho-serine 287 in Xenopus) of Cdc25, accompanied by activation of Cdc25 which would then dephosphorylate and activate Cdk1 . PP-1I has been proposed to be the form of PP-1 that dephosphorylates and activates Cdc25. Here we show that PP-1I is sequestered in a phosphorylation dependent manner by protein 14-3-3 involving phosphorylation of serine 71 of I-2 moiety of PP-1I by C-TAK1. Phosphorylation of serine 71 of the I-2 moiety of PP-1I by C-TAK1 enhanced the sequestration and inhibition of PP-1I by Protein 14-3-3. Phosphorylation of serine 71 of the I-2 moiety of PP-1I caused an increase in the Stability Energy, Binding Energy of the interaction between PP-1I and Protein 14-3-3 and a significant decrease in the IC50 of the inhibition of PP-1I by Protein 14-3-3. PP-1I regulation by C-TAK1 and Protein 14-3-3 is a mechanism for the control of Cdc25 at the G2 to M transition of the cell cycle.

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            Author and article information

            Journal
            ScienceOpen Preprints
            ScienceOpen
            9 March 2021
            Affiliations
            [1 ] Nacbraht Biomedical Research Institute
            Article
            10.14293/S2199-1006.1.SOR-.PP5HLQD.v1
            0ef19499-10b0-4918-9f3d-64eb616e61cc

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .


            The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
            Biochemistry,Life sciences
            Protein phosphatase-1 (PP-1),Protein phosphatase-1I (PP-1I),C-TAK1,Protein 14-3-3,Phosphorylation dependent sequestration,Cdc25,Meiotic and Mitotic cell division; ,G2 to M transition

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