<b>Analysis of Human Genes Associated with Ebola using Online Bioinformatics Tools</b>

Ebola is a deadlydisease caused by a virus in the family of Filoviridae. The virion is a single-stranded RNA of negative polarity and proteins enclosed in a lipid membrane. This ebolavirus species in African countries has caused several severe epidemics. Its infection causes a rapidly fatal hemorrhagic fever in humans for which no approved antivirals are available. Currently, the diagnostic tests for this deadly disease are Enzyme-Linked Immunoassay (ELISA) and Polymerase Chain Reaction (PCR) tests; viral cultivation and biopsy samples may also be used. So, there is no standard treatment for Ebola hemorrhagic fever and only supportive therapy, and experimental treatment is available. We used online bioinformatics tools to understand and look for new therapeutic targets.

Content

Author and article information

Journal

Title: ScienceOpen Preprints

Publisher: ScienceOpen

Publication date (Electronic preprint): 21 December 2022

Affiliations

[1 ] Institute of life sciences and biomedicine, Far Eastern Federal University

[2 ] Institute of biotechnology,bioengineering and food systems, Far Eastern Federal University

Author notes

[* ]Email: rahmarabia230@ 123456gmail.com .

Author information

Rahma Mohamed Bachir https://orcid.org/0000-0001-9713-2613

Article

DOI: 10.14293/S2199-1006.1.SOR-.PP6UGIK.v1

SO-VID: 58bb5569-6696-4ab7-8866-3c3600e21de9

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This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

History

Date received : 21 December 2022

References

Kuhn Jens H., Amarasinghe Gaya K., Basler Christopher F., Bavari Sina, Bukreyev Alexander, Chandran Kartik, Crozier Ian, Dolnik Olga, Dye John M., Formenty Pierre B. H., Griffiths Anthony, Hewson Roger, Kobinger Gary P., Leroy Eric M., Mühlberger Elke, Netesov (Нетёсов Сергей Викторович) Sergey V., Palacios Gustavo, Pályi Bernadett, Pawęska Janusz T., Smither Sophie J., Takada (高田礼人) Ayato, Towner Jonathan S., Wahl Victoria. ICTV Virus Taxonomy Profile: Filoviridae. Journal of General Virology. Vol. 100(6):911–912. 2019. Microbiology Society. [Cross Ref]
Bodmer Bianca S., Hoenen Thomas. Assessment of Life Cycle Modeling Systems as Prediction Tools for a Possible Attenuation of Recombinant Ebola Viruses. Viruses. Vol. 14(5)2022. MDPI AG. [Cross Ref]
Kamdar Maulik R., Dumontier Michel. An Ebola virus-centered knowledge base. Database. Vol. 2015:2015. Oxford University Press (OUP). [Cross Ref]
Wang Bin, Zhang Jing, Liu Xin, Chai Qingqing, Lu Xiaoran, Yao Xiaoyu, Yang Zhichang, Sun Liangliang, Johnson Silas F., Schwartz Richard C, Zheng Yong-Hui. Protein disulfide isomerases (PDIs) negatively regulate ebolavirus structural glycoprotein expression in the endoplasmic reticulum (ER) via the autophagy-lysosomal pathway. Autophagy. Vol. 18(10):2350–2367. 2022. Informa UK Limited. [Cross Ref]
Crozier Ian, Britson Kyla A., Wolfe Daniel N., Klena John D., Hensley Lisa E., Lee John S., Wolfraim Larry A., Taylor Kimberly L., Higgs Elizabeth S., Montgomery Joel M., Martins Karen A.. The Evolution of Medical Countermeasures for Ebola Virus Disease: Lessons Learned and Next Steps. Vaccines. Vol. 10(8)2022. MDPI AG. [Cross Ref]
Balmith Marissa, Faya Mbuso, Soliman Mahmoud E. S.. Ebola virus: A gap in drug design and discovery - experimental and computational perspective. Chemical Biology & Drug Design. Vol. 89(3):297–308. 2017. Wiley. [Cross Ref]
Feldmann Heinz, Geisbert Thomas W. Ebola haemorrhagic fever. The Lancet. Vol. 377(9768):849–862. 2011. Elsevier BV. [Cross Ref]
Feldmann Heinz. Ebola — A Growing Threat? New England Journal of Medicine. Vol. 371(15):1375–1378. 2014. Massachusetts Medical Society. [Cross Ref]
Murray Michael J.. Ebola Virus Disease. Anesthesia & Analgesia. Vol. 121(3):798–809. 2015. Ovid Technologies (Wolters Kluwer Health). [Cross Ref]
Hoenen Thomas, Groseth Allison, Falzarano Darryl, Feldmann Heinz. Ebola virus: unravelling pathogenesis to combat a deadly disease. Trends in Molecular Medicine. Vol. 12(5):206–215. 2006. Elsevier BV. [Cross Ref]
Khan Ali S., Tshioko F. Kweteminga, Heymann David L., Le Guenno Bernard, Nabeth Pierre, Kerstiëns Barbara, Fleerackers Yon, Kilmarx Peter H., Rodier Guenael R., Nkuku Okumi, Rollin Pierre E., Sanchez Anthony, Zaki Sherif R., Swanepoel Robert, Tomori Oyewale, Nichol Stuart T., Peters C. J., Muyembe‐Tamfum J. J., Ksiazek Thomas G.. The Reemergence of Ebola Hemorrhagic Fever, Democratic Republic of the Congo, 1995. The Journal of Infectious Diseases. Vol. 179(s1)1999. Oxford University Press (OUP). [Cross Ref]
Yazdanpanah Yazdan, Arribas Jose Ramon, Malvy Denis. Treatment of Ebola virus disease. Intensive Care Medicine. Vol. 41(1):115–117. 2015. Springer Science and Business Media LLC. [Cross Ref]
Bukreyev Alexander A., Chandran Kartik, Dolnik Olga, Dye John M., Ebihara Hideki, Leroy Eric M., Mühlberger Elke, Netesov Sergey V., Patterson Jean L., Paweska Janusz T., Saphire Erica Ollmann, Smither Sophie J., Takada Ayato, Towner Jonathan S., Volchkov Viktor E., Warren Travis K., Kuhn Jens H.. Discussions and decisions of the 2012–2014 International Committee on Taxonomy of Viruses (ICTV) Filoviridae Study Group, January 2012–June 2013. Archives of Virology. Vol. 159(4):821–830. 2014. Springer Science and Business Media LLC. [Cross Ref]
Baden Lindsey R., Kanapathipillai Rupa, Campion Edward W., Morrissey Stephen, Rubin Eric J., Drazen Jeffrey M.. Ebola — An Ongoing Crisis. New England Journal of Medicine. Vol. 371(15):1458–1459. 2014. Massachusetts Medical Society. [Cross Ref]
Fauci Anthony S.. Ebola — Underscoring the Global Disparities in Health Care Resources. New England Journal of Medicine. Vol. 371(12):1084–1086. 2014. Massachusetts Medical Society. [Cross Ref]
Swetha Rayapadi G., Ramaiah Sudha, Anbarasu Anand, Sekar Kanagaraj. <i>Ebolavirus</i> Database: Gene and Protein Information Resource for Ebolaviruses. Advances in Bioinformatics. Vol. 2016:1–4. 2016. Hindawi Limited. [Cross Ref]
Stein Lincoln D., Mungall Christopher, Shu ShengQiang, Caudy Michael, Mangone Marco, Day Allen, Nickerson Elizabeth, Stajich Jason E., Harris Todd W., Arva Adrian, Lewis Suzanna. The Generic Genome Browser: A Building Block for a Model Organism System Database. Genome Research. Vol. 12(10):1599–1610. 2002. Cold Spring Harbor Laboratory. [Cross Ref]
Frieden Thomas R., Damon Inger, Bell Beth P., Kenyon Thomas, Nichol Stuart. Ebola 2014 — New Challenges, New Global Response and Responsibility. New England Journal of Medicine. Vol. 371(13):1177–1180. 2014. Massachusetts Medical Society. [Cross Ref]
Oluwagbemi Olugbenga, Awe Olaitan. A comparative computational genomics of Ebola Virus Disease strains: In-silico Insight for Ebola control. Informatics in Medicine Unlocked. Vol. 12:106–119. 2018. Elsevier BV. [Cross Ref]

Comments

Comment on this article

[1] Kuhn Jens H., Amarasinghe Gaya K., Basler Christopher F., Bavari Sina, Bukreyev Alexander, Chandran Kartik, Crozier Ian, Dolnik Olga, Dye John M., Formenty Pierre B. H., Griffiths Anthony, Hewson Roger, Kobinger Gary P., Leroy Eric M., Mühlberger Elke, Netesov (Нетёсов Сергей Викторович) Sergey V., Palacios Gustavo, Pályi Bernadett, Pawęska Janusz T., Smither Sophie J., Takada (高田礼人) Ayato, Towner Jonathan S., Wahl Victoria. ICTV Virus Taxonomy Profile: Filoviridae. Journal of General Virology. Vol. 100(6):911–912. 2019. Microbiology Society. [Cross Ref]

[2] Bodmer Bianca S., Hoenen Thomas. Assessment of Life Cycle Modeling Systems as Prediction Tools for a Possible Attenuation of Recombinant Ebola Viruses. Viruses. Vol. 14(5)2022. MDPI AG. [Cross Ref]

[3] Kamdar Maulik R., Dumontier Michel. An Ebola virus-centered knowledge base. Database. Vol. 2015:2015. Oxford University Press (OUP). [Cross Ref]

[4] Wang Bin, Zhang Jing, Liu Xin, Chai Qingqing, Lu Xiaoran, Yao Xiaoyu, Yang Zhichang, Sun Liangliang, Johnson Silas F., Schwartz Richard C, Zheng Yong-Hui. Protein disulfide isomerases (PDIs) negatively regulate ebolavirus structural glycoprotein expression in the endoplasmic reticulum (ER) via the autophagy-lysosomal pathway. Autophagy. Vol. 18(10):2350–2367. 2022. Informa UK Limited. [Cross Ref]

[5] Crozier Ian, Britson Kyla A., Wolfe Daniel N., Klena John D., Hensley Lisa E., Lee John S., Wolfraim Larry A., Taylor Kimberly L., Higgs Elizabeth S., Montgomery Joel M., Martins Karen A.. The Evolution of Medical Countermeasures for Ebola Virus Disease: Lessons Learned and Next Steps. Vaccines. Vol. 10(8)2022. MDPI AG. [Cross Ref]

[6] Balmith Marissa, Faya Mbuso, Soliman Mahmoud E. S.. Ebola virus: A gap in drug design and discovery - experimental and computational perspective. Chemical Biology & Drug Design. Vol. 89(3):297–308. 2017. Wiley. [Cross Ref]

[7] Feldmann Heinz, Geisbert Thomas W. Ebola haemorrhagic fever. The Lancet. Vol. 377(9768):849–862. 2011. Elsevier BV. [Cross Ref]

[8] Feldmann Heinz. Ebola — A Growing Threat? New England Journal of Medicine. Vol. 371(15):1375–1378. 2014. Massachusetts Medical Society. [Cross Ref]

[9] Murray Michael J.. Ebola Virus Disease. Anesthesia & Analgesia. Vol. 121(3):798–809. 2015. Ovid Technologies (Wolters Kluwer Health). [Cross Ref]

[10] Hoenen Thomas, Groseth Allison, Falzarano Darryl, Feldmann Heinz. Ebola virus: unravelling pathogenesis to combat a deadly disease. Trends in Molecular Medicine. Vol. 12(5):206–215. 2006. Elsevier BV. [Cross Ref]

[11] Khan Ali S., Tshioko F. Kweteminga, Heymann David L., Le Guenno Bernard, Nabeth Pierre, Kerstiëns Barbara, Fleerackers Yon, Kilmarx Peter H., Rodier Guenael R., Nkuku Okumi, Rollin Pierre E., Sanchez Anthony, Zaki Sherif R., Swanepoel Robert, Tomori Oyewale, Nichol Stuart T., Peters C. J., Muyembe‐Tamfum J. J., Ksiazek Thomas G.. The Reemergence of Ebola Hemorrhagic Fever, Democratic Republic of the Congo, 1995. The Journal of Infectious Diseases. Vol. 179(s1)1999. Oxford University Press (OUP). [Cross Ref]

[12] Yazdanpanah Yazdan, Arribas Jose Ramon, Malvy Denis. Treatment of Ebola virus disease. Intensive Care Medicine. Vol. 41(1):115–117. 2015. Springer Science and Business Media LLC. [Cross Ref]

[13] Bukreyev Alexander A., Chandran Kartik, Dolnik Olga, Dye John M., Ebihara Hideki, Leroy Eric M., Mühlberger Elke, Netesov Sergey V., Patterson Jean L., Paweska Janusz T., Saphire Erica Ollmann, Smither Sophie J., Takada Ayato, Towner Jonathan S., Volchkov Viktor E., Warren Travis K., Kuhn Jens H.. Discussions and decisions of the 2012–2014 International Committee on Taxonomy of Viruses (ICTV) Filoviridae Study Group, January 2012–June 2013. Archives of Virology. Vol. 159(4):821–830. 2014. Springer Science and Business Media LLC. [Cross Ref]

[14] Baden Lindsey R., Kanapathipillai Rupa, Campion Edward W., Morrissey Stephen, Rubin Eric J., Drazen Jeffrey M.. Ebola — An Ongoing Crisis. New England Journal of Medicine. Vol. 371(15):1458–1459. 2014. Massachusetts Medical Society. [Cross Ref]

[15] Fauci Anthony S.. Ebola — Underscoring the Global Disparities in Health Care Resources. New England Journal of Medicine. Vol. 371(12):1084–1086. 2014. Massachusetts Medical Society. [Cross Ref]

[16] Swetha Rayapadi G., Ramaiah Sudha, Anbarasu Anand, Sekar Kanagaraj. <i>Ebolavirus</i> Database: Gene and Protein Information Resource for Ebolaviruses. Advances in Bioinformatics. Vol. 2016:1–4. 2016. Hindawi Limited. [Cross Ref]

[17] Stein Lincoln D., Mungall Christopher, Shu ShengQiang, Caudy Michael, Mangone Marco, Day Allen, Nickerson Elizabeth, Stajich Jason E., Harris Todd W., Arva Adrian, Lewis Suzanna. The Generic Genome Browser: A Building Block for a Model Organism System Database. Genome Research. Vol. 12(10):1599–1610. 2002. Cold Spring Harbor Laboratory. [Cross Ref]

[18] Frieden Thomas R., Damon Inger, Bell Beth P., Kenyon Thomas, Nichol Stuart. Ebola 2014 — New Challenges, New Global Response and Responsibility. New England Journal of Medicine. Vol. 371(13):1177–1180. 2014. Massachusetts Medical Society. [Cross Ref]

[19] Oluwagbemi Olugbenga, Awe Olaitan. A comparative computational genomics of Ebola Virus Disease strains: In-silico Insight for Ebola control. Informatics in Medicine Unlocked. Vol. 12:106–119. 2018. Elsevier BV. [Cross Ref]

Analysis of Human Genes Associated with Ebola using Online Bioinformatics Tools