Molecularly imprinted polymers (MIPs), are materials that has been processed using the molecular imprinting technique. These technology involves three strategies, that is, covalent, non-covalent, and semi-covalent approach (non-covalent approach is commonly used). The most important component for the synthesis of MIPs are template, functional monomer, cross-linkers, initiators and porogenic solvent. Molecularly imprinted polymers have been used in diverse ways, some of which includes; application as polymeric sensors, toxicant selective polymers, drug delivery system (DDS), ocular therapy, Molecular imprinted polymer has been applied in the treatment of contagious tropical infections, and MIP in transdermal therapeutic applications. In this study, an ultra violet visible investigation of the optimal reacting ratio for nicotine and Pleurotus tuberregium was carried out. At 1:5 and 1:6 nicotine and Pleurotus tuberregium ratio, the absorbance was 0.352nm and 0.354nm respectively. 1:5 and 1:6 are suitable ratios for template functional monomer interaction due to their stability. Over times, synthetic monomers are commonly used for MIPs, however, more studies should be carried out with biopolymers which are easily available, low cost and easily degradable.