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      Pitfalls at Chemistry of Adenoviral Vector Vaccine against COVID-19 and how to Circumvent It.

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            Abstract

            ChAdOx1 nCoV-19 vaccine is an adenovirus vector vaccine that designed to provoke immunity against SARS-CoV-2.This vaccine contains several inactive ingredients, including sodium chloride, magnesium chloride hexahydrate, ethanol, sucrose, and Ethylenediaminetetraacetic acid (EDTA).EDTA is a very potent zinc chelator which is used commonly in protein interaction studies. Exposure to EDTA even in lower concentrations may cause extreme stripping of zinc from many proteins, in-cluding zinc-binding proteins that described as a component of the largest and most complex gene superfamily in metazoans and the most common class of transcription factors.the EDTA-induced thrombocytopenia is a risk phenomenon caused by EDTA-dependent anti-platelet auto-antibodies that identify antigens modified by EDTA.Another issue is the adenovirus in the vaccine. It can induce thrombocytopenia, a potentially serious complication of gene therapy protocols using this type of vector.PEGylation already induced significantly lower serum il-6 levels by 70% (14)We noted that the study by Katie et al studied immune response by a single dose of ChAdOx1 nCov-19 not included test of Il-6 (15)We conclude from previous studies that the PEGylating of adenoviral vectors can be promise tech-nology as safety profile as significantly reduced IL-6 and liver toxicity and how avoiding the pitfalls of chemistry and virology so the PEGylation since first time at 1999 introduced by O’Riordan,C.R et al (16) need more advancements

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            Author and article information

            Journal
            ScienceOpen Preprints
            ScienceOpen
            27 June 2021
            Affiliations
            [1 ] 1Department of Public Health, Tuberculosis Program, First Health Cluster, Ministry of Health, Riyadh, Saudi Arabia.
            [2 ] 3Biomedical sciences department, Faculty of Veterinary Medicine, King Faisal University, Saudi Arabia.
            Author notes
            Article
            10.14293/S2199-1006.1.SOR-.PP96ICB.v1
            b41596cb-668f-4ee2-a517-fda728b93614

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .


            The datasets generated during and/or analysed during the current study are available in the repository: http://10.31219/osf.io/anqcp
            Chemistry,Pharmacology & Pharmaceutical medicine,Infectious disease & Microbiology
            : ChAdOx1 COVID-19 vaccine; EDTA; zinc; adenovirus; polyethylene glycol

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