Pitfalls at Chemistry of Adenoviral Vector Vaccine against COVID-19 and how to Circumvent It.

ChAdOx1 nCoV-19 vaccine is an adenovirus vector vaccine that designed to provoke immunity against SARS-CoV-2.This vaccine contains several inactive ingredients, including sodium chloride, magnesium chloride hexahydrate, ethanol, sucrose, and Ethylenediaminetetraacetic acid (EDTA).EDTA is a very potent zinc chelator which is used commonly in protein interaction studies. Exposure to EDTA even in lower concentrations may cause extreme stripping of zinc from many proteins, in-cluding zinc-binding proteins that described as a component of the largest and most complex gene superfamily in metazoans and the most common class of transcription factors.the EDTA-induced thrombocytopenia is a risk phenomenon caused by EDTA-dependent anti-platelet auto-antibodies that identify antigens modified by EDTA.Another issue is the adenovirus in the vaccine. It can induce thrombocytopenia, a potentially serious complication of gene therapy protocols using this type of vector.PEGylation already induced significantly lower serum il-6 levels by 70% (14)We noted that the study by Katie et al studied immune response by a single dose of ChAdOx1 nCov-19 not included test of Il-6 (15)We conclude from previous studies that the PEGylating of adenoviral vectors can be promise tech-nology as safety profile as significantly reduced IL-6 and liver toxicity and how avoiding the pitfalls of chemistry and virology so the PEGylation since first time at 1999 introduced by O’Riordan,C.R et al (16) need more advancements