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      Identification of CYP2D6 allelic mutations in a sub-set of Karachi population

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      CYP2D6 alleles, Polymorphism, Depression, Drug metabolism, Karachi population

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          Abstract

          CYP2D6 gene polymorphism is considered a main obstacle in the process of drug metabolism under clinical diseases that affect pharmacokinetics of ~25% of antidepressants and other drugs. Inter-individual variation occurs in the amount and functional activity of CYP2D6 enzyme produce undesirable side effects. The primary aim of current research is to evaluate gene and genotypic frequencies of CYP2D6 *1 extensive metabolizer, *4 poor metabolizer and *10 intermediate metabolizer allelic variants among depressed patients and compared with normal subjects and other populations. Human genomic DNA was isolated. Genotyping was performed by Polymerase chain reaction followed by restriction endonucleases digestion for variants analysis. The results indicated gene frequency of CYP2D6*1 was 59% (CI 49.6,68.3%) in normal subjects whereas, CYP2D6*4 was 13% (CI 3.7, 22.3%) and CYP2D6*10 was 54% (44.7, 63.3%) predominantly found in depressed patients. The results demonstrate pronounced association of CYP2D6 *4 and *10 allelic variants with patient’s drug response activity.

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          Author and article information

          Journal
          ScienceOpen Preprints
          ScienceOpen
          26 March 2021
          Affiliations
          [1 ] Dr. Panjwani Center for Molecular Medicine and Drug Research
          Article
          10.14293/S2199-1006.1.SOR-.PPAE8YV.v1

          This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

          All data generated or analysed during this study are included in this published article (and its supplementary information files).

          Life sciences

          Karachi population, Drug metabolism, Depression, Polymorphism, CYP2D6 alleles

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