Verifying Primers For Point Mutation Using Primers To Amplify DNA Region Containing Mutation Site

Analysing DNA sequences for determining mutation point in PCR needs paid, Hi-tech software tools but we can confidently order the right piece of primer by this simple and easy method before we do PCR in lab. For instance, we here take BRAF V600E mutation. This method is to verify that the primers you’ve got or designed would successfully amplify the same region of DNA you want and not somewhere else.

Content

Author and article information

Journal

Title: ScienceOpen Preprints

Publisher: ScienceOpen

Publication date (Electronic preprint): 28 June 2021

Affiliations

[1 ] Student, Government Biotech College, INDIA.

Author notes

[* ]Email: halakshukla0101@ 123456gmail.com .

Author information

Halak Shukla https://orcid.org/0000-0002-6041-3793

Article

DOI: 10.14293/S2199-1006.1.SOR-.PPBAGT6.v1

SO-VID: 16f0a652-7102-4925-aa98-ea4dce4478af

License:

This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

History

Date received : 28 June 2021

Funding

N/A N/A

Data availability: All data generated or analysed during this study are included in this published article (and its supplementary information files).

ScienceOpen disciplines: Bioinformatics & Computational biology,Biotechnology

Keywords: PCR,BRAF,BRAF V600E,Point mutation ,Trick method,Primers,Easy tools,Save costs ,Dry lab,Amplify DNA sequence