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      Verifying Primers For Point Mutation Using Primers To Amplify DNA Region Containing Mutation Site

      Preprint
      In review
      research-article
        1 ,
      ScienceOpen Preprints
      ScienceOpen
      PCR, BRAF, BRAF V600E, Point mutation , Trick method, Primers, Easy tools, Save costs , Dry lab, Amplify DNA sequence
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            Abstract

            Analysing DNA sequences for determining mutation point in PCR needs paid, Hi-tech software tools but we can confidently order the right piece of primer by this simple and easy method before we do PCR in lab. For instance, we here take BRAF V600E mutation. This method is to verify that the primers you’ve got or designed would successfully amplify the same region of DNA you want and not somewhere else.

            Content

            Author and article information

            Journal
            ScienceOpen Preprints
            ScienceOpen
            28 June 2021
            Affiliations
            [1 ] Student, Government Biotech College, INDIA.
            Author notes
            Article
            10.14293/S2199-1006.1.SOR-.PPBAGT6.v1
            16f0a652-7102-4925-aa98-ea4dce4478af

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

            Funding
            N/A N/A

            All data generated or analysed during this study are included in this published article (and its supplementary information files).
            Bioinformatics & Computational biology,Biotechnology
            PCR,BRAF,BRAF V600E,Point mutation ,Trick method,Primers,Easy tools,Save costs ,Dry lab,Amplify DNA sequence

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