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      Chalcone Synthase: Method Optimization of Extraction and Activity Assay in Strawberry Fruit

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          Abstract

          Method development and optimization play a central role in analytical chemistry and more specifically in food biochemistry. When it comes to research, it is common that analytical methods need to be modified to specific experimental biological tissues. While there are several published works on the activity of the enzyme chalcone synthase (CHS) in plant materials, such as sweet basil using ultra- high-performance liquid chromatography, there is a lack of information regarding extraction and activity of CHS in strawberries. Therefore, the main objective of this work was to optimize existing published methods for extraction and activity of CHS in strawberries, using spectrophotometric analysis. It was done through a literature search, a method dissection was performed, followed by theoretical optimization of the protocol, and finally an experimental optimization

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          Author and article information

          Journal
          ScienceOpen Posters
          ScienceOpen
          28 July 2020
          Affiliations
          [1 ] Food Quality Laboratory, Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, USA
          Article
          10.14293/S2199-1006.1.SOR-.PPCYRQC.v1

          This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

          All data generated or analysed during this study are included in this published article (and its supplementary information files).

          Chemistry, Life sciences

          Food Biochemistry23, Optimization, Chalcone Synthase, Strawberry Fruit

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