Antimicrobial resistance has become a serious problem for physicians around the world when it comes to treating patients. As a result, short-term research was carried out by us to determine the prevalence of multidrug-resistant (MDR) bacterial isolates and to study the mechanism of escaping the antibiotics. Methods and Materials In the department of microbiology, clinical samples were grown and bacterial strains were discovered. To determine we have studied several strains of clinically established MDR bacteria and analyzed their genome sequences to get the answers to the tolerance and resistance of MDR bacteria.