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      PAX6: a transcriptional guardian for glutamatergic fate in the developing neocortex

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            Abstract

            During forebrain development, the transcription factor PAX6 is highly expressed by progenitors in the dorsal telencephalon (dTel) i.e. the primitive cerebral cortex with a sharp boundary at the pallial-subpallial boundary, thereby establishing the dorso-ventral patterning of the forebrain and regulating the generation of cortical glutamatergic neurons. Strikingly, removal of Pax6 led to a diversion away from the glutamatergic identity in a subset of cortical progenitors indicated by ectopic gene expression. We postulate that PAX6 confers glutamatergic fate in progenitors by preventing them from responding to signaling cues such as SHH that can induce abberant fates. In the present study, we used the transgenic mouse model with Pax6 conditionally deleted in the cortex using a tamoxifen-inducible Emx1-CreER T2 transgene combined with a floxed Pax6 and an EGFP constructs. Single-cell transcriptome revealed multiple ectopic leanages in cortical progenitors with morphogen-regulated transcriptional signatures upon Pax6 deletion. We also undertook a candidate approach to investigate how attenuation of signaling cues by surgical and pharmacological means using in vitro slice culture affect the magnitude of ectopic gene expression in the cortical progenitors. We demonstrated that attenuation of interneuron migration into the cortex and inhibition of SHH signaling pathway in slice culture substatially reduced aberrant gene expression in the cortical progenitors. Our findings suggest that ventral cues from vTel such as SHH possess ventralizing effect on cortical progenitors, this is consistent with a requirement for PAX6 to resist such effects in order to safeguard glutamatergic fate.

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            Author and article information

            Journal
            ScienceOpen Posters
            ScienceOpen
            22 February 2021
            Affiliations
            [1 ] Centre for Discovery Brain Sciences (CDBS), University of Edinburgh, Edinburgh, United Kingdom.
            Author information
            https://orcid.org/0000-0002-4321-0193
            https://orcid.org/0000-0001-7674-8785
            https://orcid.org/0000-0002-0489-2400
            https://orcid.org/0000-0002-5318-307X
            Article
            10.14293/S2199-1006.1.SOR-.PPKAD1S.v2
            a5c05002-041b-476e-ad16-1f6ab8332a51

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

            History
            : 10 July 2020

            The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
            Medicine,Life sciences
            pax6,neurogenesis,cell fate,fate decision,glutamatergic neurons,fate specification,neocortex development,developing forebrain

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