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AMPLIFICATION AND SEQUENCE ANALYSIS OF dhaS, ONE COMPONENT OF THE INDOLE-3-PYRUVIC ACID SYNTHETIC PATHWAY OF THE PHYTOHORMONE INDOLE-3-ACETIC ACID
Abstract
Several species of Bacillus are plant growth-promoting rhizobacteria that can produce the phytohormone Indole-3-acetic acid (IAA) which regulates plant growth and development, and in some species, protects the host plant from pathogen invasion. Previous studies reveal several pathways for IAA biosynthesis in various bacterial species including genes and enzymes that take part in the biosynthetic pathway. In this study, we aim to amplify dhaS, one of the component genes from the indole-3-pyruvic acid pathway (IPyA) for IAA synthesis, and to conduct 16S rRNA sequence analysis from two Bacillus spp. isolated from biofertilizer. Seqeuncing of the dhaS gene amplified through polymerase chain reaction using designed gene specific primers showed 98% identity with B. velezensis strain FJAT-45028 and a translated BLAST hit with an aldehyde dehydrogenase protein, having percent identity of 96%. Secondary structure of the protein coded by dhaS gene in isolate 1 displayed models of proline dehydrogenase and aldehyde dehydrogenase. Furthermore, 16s rRNA sequences from two Bacillus isolates were analyzed. 16S rRNA from Isolate 1 showed a top BLAST hit with Bacillus sp. strain 1CY1 (99.67%) while Isolate 2 showed a top BLAST hit with B.subtilis strain GX S-11 (95.65%). Phylogenetic trees were generated to reveal the relationship of the two isolates to their top five BLAST hits. To further understand the potential roles of dhaS in the IPyA pathway for IAA synthesis, transcriptional responses to l-tryptophan and functional genomic studies must be done. These will help us further understand the physiological bases of biofertilizers towards sustainable agriculture.
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Kelley Lawrence A, Mezulis Stefans, Yates Christopher M, Wass Mark N, Sternberg Michael J E. The Phyre2 web portal for protein modeling, prediction and analysis. Nature Protocols. Vol. 10(6):845–858. 2015. Springer Science and Business Media LLC. [Cross Ref]