<b>AMPLIFICATION AND SEQUENCE ANALYSIS OF dhaS, ONE COMPONENT OF THE INDOLE-3-PYRUVIC ACID SYNTHETIC PATHWAY </b> <b>OF THE PHYTOHORMONE INDOLE-3-ACETIC ACID</b>

Several species of Bacillus are plant growth-promoting rhizobacteria that can produce the phytohormone Indole-3-acetic acid (IAA) which regulates plant growth and development, and in some species, protects the host plant from pathogen invasion. Previous studies reveal several pathways for IAA biosynthesis in various bacterial species including genes and enzymes that take part in the biosynthetic pathway. In this study, we aim to amplify dhaS, one of the component genes from the indole-3-pyruvic acid pathway (IPyA) for IAA synthesis, and to conduct 16S rRNA sequence analysis from two Bacillus spp. isolated from biofertilizer. Seqeuncing of the dhaS gene amplified through polymerase chain reaction using designed gene specific primers showed 98% identity with B. velezensis strain FJAT-45028 and a translated BLAST hit with an aldehyde dehydrogenase protein, having percent identity of 96%. Secondary structure of the protein coded by dhaS gene in isolate 1 displayed models of proline dehydrogenase and aldehyde dehydrogenase. Furthermore, 16s rRNA sequences from two Bacillus isolates were analyzed. 16S rRNA from Isolate 1 showed a top BLAST hit with Bacillus sp. strain 1CY1 (99.67%) while Isolate 2 showed a top BLAST hit with B.subtilis strain GX S-11 (95.65%). Phylogenetic trees were generated to reveal the relationship of the two isolates to their top five BLAST hits. To further understand the potential roles of dhaS in the IPyA pathway for IAA synthesis, transcriptional responses to l-tryptophan and functional genomic studies must be done. These will help us further understand the physiological bases of biofertilizers towards sustainable agriculture.

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Author and article information

Journal

Title: ScienceOpen Posters

Publisher: ScienceOpen

Publication date (Electronic preprint): 27 February 2022

Affiliations

[1 ] Institute of Weed Science, Entomology and Plant Pathology, Colloge of Agriculture and Food Science, University of the Philippines Los Banos

[2 ] Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Banos

[3 ] National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Banos

Author notes

[* ]Email: kbalviar@ 123456up.edu.ph .

Author information

Karen Alviar https://orcid.org/0000-0002-6759-0100

Article

DOI: 10.14293/S2199-1006.1.SOR-.PPMUMHR.v1

SO-VID: 8ea14f21-4e7e-4c45-b97d-c0ffae548b7e

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This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

History

Date received : 27 February 2022

Data availability: The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

ScienceOpen disciplines: Agriculture,Chemistry,Life sciences

Keywords: IAA,dhaS,biofertilizer,Bacillus spp.,16s rRNA,IPyA

References

Kelley Lawrence A, Mezulis Stefans, Yates Christopher M, Wass Mark N, Sternberg Michael J E. The Phyre2 web portal for protein modeling, prediction and analysis. Nature Protocols. Vol. 10(6):845–858. 2015. Springer Science and Business Media LLC. [Cross Ref]

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[1] Kelley Lawrence A, Mezulis Stefans, Yates Christopher M, Wass Mark N, Sternberg Michael J E. The Phyre2 web portal for protein modeling, prediction and analysis. Nature Protocols. Vol. 10(6):845–858. 2015. Springer Science and Business Media LLC. [Cross Ref]