Quantitative Trait Locus Mapping of Host-plant
Resistance against Insect Pests

Host-plant resistance is a fundamental part of integrated pest management through breeding for genes conferring resistance against insect pests. One method that can achieve host plant resistance to insects is through quantitative trait locus (QTL) mapping analysis. This method has advanced significantly recently because of the availability of modern genomic methods and instrumentation, powerful statistical methods, and wide range of molecular markers. Single nucleotide polymorphism (SNPs) and genotyping by sequence (GBS) resulted in high through-put and cost-effective genotyping. Integration of GBS and biparental mapping is a powerful tool as it can be adapted to species that lack preliminary sequence or genotypic information. QTL analysis should be used in untargeted approach and aimed for combined-insect resistance genomic studies because insect pests induce damage in many different ways. Combining QTL mapping analyses with transcriptomics, proteomics and metabolomics, to assess insect resistance at the levels of the genotype, gene expression, and metabolite and protein networks is an integral part to produce multiple pest-resistance breeding.