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      Ginsenoside Rg1 attenuates arsenic-induced mice nephrotoxicity via the activated HO-1/mTOR-associated apoptosis or autophagy signaling

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      ScienceOpen Preprints
      ScienceOpen
      Arsenic, Nephrotoxicity, Ginsenoside Rg1, Apoptosis, Autophagy
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            Abstract

            Nephrotoxicity attributed to environmental arsenic exposure, has been recognized by animal experiments and populational survey over 30 years in China, given a significance of public health by preventing from the disorder of renal function and hispathological abnormality. Here, Ginsenoside Rg1 (Rg1) as the commercial bioactive product of ginseng, play a beneficial role via antioxidant, anti-inflammatory and anti-apoptotic effects, which is poorly understood in arsenic-induced nephrotoxicity. The present study applied animal experiments to explore the pharmacological effects of Rg1 on sodium arsenite (SA)-induced nephrotoxicity in mice. Results showed that SA exposure led to renal pathological damage, and induced renal oxidative stress and the elevated levels of apoptosis or autophagy-associated indices in kidney. Further, western-blotting results confirmed the upregulations of pro-apoptotic Bax or autophagic unc-51-like kinase-1 (ULK1) or LC3-B signal, and the downregulations of HO-1 or mTOR signal and autophagy substrate sequestosome 1 (p62/SQSTM1) in kidney. Significantly, the intervention with Rg1 alleviated arsenic-induced renal pathological damage and oxidative stress, and upregulated the levels of HO-1, mTOR and p62, while the levels of Bax, ULK1 or LC3-B downregulated in kidney. In conclusion, the intervention with Rg1 relieves arsenic-induced mice nephrotoxicity maybe involved in the regulation of HO-1/mTOR-related apoptotic or autophagic signaling.

            Content

            Author and article information

            Journal
            ScienceOpen Preprints
            ScienceOpen
            17 May 2021
            Affiliations
            [1 ] The First Affiliated Hospital of Hunan University of Medicine, Hunan University of Medicine
            Article
            10.14293/S2199-1006.1.SOR-.PPSYGZI.v1
            58169ca2-8e9d-45e3-9b03-cf54c6dab71c

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

            Funding
            Natural Science Foundations of China 81960597

            All data generated or analysed during this study are included in this published article (and its supplementary information files).
            Toxicology,Medicine,Pharmacology & Pharmaceutical medicine
            Apoptosis,Ginsenoside Rg1,Arsenic,Nephrotoxicity,Autophagy

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