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      Enhancing plant genome editing through the use of a flexible CRISPR-Combo system

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      CRISPR, Genome Engineering, CRISPR combo system, Plant genome, Genomics, Cas9
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            Abstract

            Plant genome engineering has greatly benefited from the use of CRISPR-Cas9, its derived base editors, and CRISPR activation systems. However, since these systems are typically used independently, their combined potential is largely unrealized. We create a flexible CRISPR-Combo platform for simultaneous genome editing (targeted mutagenesis or base editing) and gene activation in plants here, based on a single Cas9 protein. We highlight the effective CRISPR-Combo applications for enhancing plant genome editing. By activating the florigen gene in Arabidopsis, CRISPR-Combo is used to shorten the plant life cycle and reduce the effort required to screen transgene-free genome-edited plants. We then use the activation of morphogenic genes in poplar to show how genome-edited plants can regenerate and reproduce more quickly. Additionally, we use CRISPR-Combo, which is recognized as a new technique to primarily enrich heritable targeted mutations, to achieve rice regeneration without the use of exogenous plant hormones. As a versatile tool for genome engineering, CRISPR-Combo has potential uses in crop breeding.

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            Author and article information

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            ScienceOpen Posters
            ScienceOpen
            2 December 2022
            Affiliations
            [1 ] Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kerala, india
            Author notes
            Author information
            https://orcid.org/0000-0001-6024-1699
            Article
            10.14293/S2199-1006.1.SOR-.PPTSMD2.v1
            d753bbed-3a51-4453-99b9-1024fb2850c4

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

            History
            : 2 December 2022
            Categories

            Data sharing not applicable to this article as no datasets were generated or analysed during the current study.
            Agriculture,Chemistry,Life sciences
            CRISPR,Genome Engineering,CRISPR combo system,Plant genome,Genomics,Cas9

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