Mitochondrial Dynamics: impact on adult neural stem cell fate

Abstract

Neural stem cells (NSCs) are found in discrete regions of the mammalian brain. During adulthood, NSCs can be a source of new neurons and oligodendrocytes in neurological disorders. However, these newborn cells are not sufficient to overcome the neurological deficits involved by neural loss. Therefore, the identification of novel mechanisms responsible for modulating NSC fate represent a major key issue for future brain repair strategies. Several studies suggest that mitochondria have an important role in regulating NSC differentiation and lineage determination. However, the molecular mechanisms involved in this regulation remain unknown. Hence, our work aims to dissect how mitochondria biogenesis and dynamics can modulate the NSC differentiation into neurons or oligodendrocytes. For this, NSCs were obtained by isolating subventricular zone (SVZ) and dentate gyrus (DG) cells from P1-3 mouse models. Seeding density, culture conditions and number of passages were determined. Moreover, the multipotency of SVZ/DG-derived NSPCs, obtained from different passages, was also accessed. Additionally, expression of proteins involved in mitochondrial biogenesis and fusion/fission appear altered during NSPC differentiation, while mitochondrial network revealed different morphologies in cells from different lineages. The results obtained will provide novel findings concerning the role of mitochondrial dynamics in NSC fate.

Journal

Title: ScienceOpen Posters

Publisher: ScienceOpen

Publication date (Electronic preprint): 10 July 2020

Affiliations

[1 ] Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Instituto de Farmacologia e Neurociências, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Instituto de Biologia Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

[2 ] Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Instituto de Farmacologia e Neurociências, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal

[3 ] UC Leuven Limburg, 3000 Leuven, Belgium

[4 ] Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Instituto de Biologia Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

Article

DOI: 10.14293/S2199-1006.1.SOR-.PPVI8US.v1

License:

This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .