Blog
About

179
views
1
recommends
+1 Recommend
1 collections
    2
    shares
      • Record: found
      • Abstract: found
      • Poster: found
      Is Open Access

      Decoding Parkinson Disorder (PD) Pathophysiology: α-Synuclein over-expression enhances inward calcium influx and exosome release in LUHMES cells.

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The hall-mark events for Parkinsons Disorder (PD) are Lewy Body (LB) formation and further degeneration of midbrain dopaminergic neurons. Even though α-Synuclein (SNCA) is established as key molecular component of LBs, yet there is no clear understanding of pathophysiological mechanisms acting behind the scene. In such scenario we failed to comprehensively demonstrate the exact reasons behind PD pathology seeding and its spread. Several mechanisms are proposed for PD pathology seeding and spreading and among these the transfer of pathological SNCA via exosomal/extracellular vesicles release is also advocated.We performedpatch-clamp electrophysiology recordings on fetal human mesencephalic cell line (LUHMES, Lund human mesencephalic); a mimiced model cell system of dopaminergic neurons in vitro. Voltage clamp recordings from SNCA overexpressing LUHMES cells accounts for an altered morphology, enhanced inward Ica and membrane capacitance ( Cm). NTA results suggested us a higher particle concentration for the size range between 50-150 nm, correspondingto the size of exosomes.

          Related collections

          Author and article information

          Journal
          ScienceOpen Posters
          ScienceOpen
          31 January 2020
          Affiliations
          [1 ] DZNE/TUM/LMU/Munich
          [2 ] DZNE/TUM/Munich;MHH/Hannover
          [3 ] DZNE/LMU/Munich
          Article
          10.14293/S2199-1006.1.SOR-.PPYUSNN.v1

          This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

          The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

          Medicine, Molecular medicine, Neurosciences

          Parkinsons, Exosomes, patch-clamp electrophysiology, LUHMES cells

          Comments

          Comment on this article