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Techniques for Separation and Selection of Antigen Specific Lymphocytes

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Author(s): W. Haas , H. von Boehmer

Publication date (Print): 1978

Publisher: Springer US

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Continuous cultures of fused cells secreting antibody of predefined specificity.

G Köhler, C Milstein (1975)

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A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.

J. E. TILL, E. McCulloch (1961)

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Selective in vitro growth of T lymphocytes from normal human bone marrows.

D. A. Morgan, F Ruscetti, R Gallo (1976)

Selective growth of T lymphocytes occurred when unfractionated normal human bone marrow cells were cultured with conditioned medium obtained from phytohemagglutinin-stimulated normal human lymphocytes (Ly-CM). Cultures of up to 90 percent T cells have been maintained for more than 9 months. The T cells exhibited a strict growth dependence upon Ly-CM and were consistently negative for Epstein-Barr viral information.