Humans are exposed to microorganism in most environments, including indoors. In this study, we examined the occupational exposure to bacteria and fungi and its correlation with indoor temperature and relative humidity for people employed in five nursing homes in the Capital Region of Denmark. We found negative correlations between temperature and fungal and bacterial exposures in personal air, sedimented dust, and surface samples, whereas we found positive correlations between bacteria and relative humidity for indoor and outdoor air samples. Our findings seem to match that of other studies, which have found strong correlations between temperature and relative humidity on the concentrations of microorganisms.