Molecular diversity analysis of Tetradium ruticarpum ( WuZhuYu ) in China based on inter-primer binding site (iPBS) markers and inter-simple sequence repeat (ISSR) markers
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Abstract
“ Wu zhu yu ”, which is obtained from the dried unripe fruits of Tetradium ruticarpum
(A. Jussieu) T. G. Hartley, has been used as a traditional Chinese medicine for treatment
of headaches, abdominal colic, and hypertension for thousands of years. The present
study was designed to assess the molecular genetic diversity among 25 collected accessions
of T. ruticarpum ( Wu zhu yu in Chinese) from different areas of China, based on
inter-primer binding site (iPBS) markers and inter-simple sequence repeat (ISSR) markers.
Thirteen ISSR primers generated 151 amplification bands, of which 130 were polymorphic.
Out of 165 bands that were amplified using 10 iPBS primers, 152 were polymorphic.
The iPBS markers displayed a higher proportion of polymorphic loci (PPL = 92.5%) than
the ISSR markers (PPL = 84.9%). The results showed that T. ruticarpum possessed
high loci polymorphism and genetic differentiation occurred in this plant. The combined
data of iPBS and ISSR markers scored on 25 accessions produced five clusters that
approximately matched the geographic distribution of the species. The results indicated
that both iPBS and ISSR markers were reliable and effective tools for analyzing the
genetic diversity in T. ruticarpum .
Author and article information
Journal
Title:
Chinese Journal of Natural Medicines
Abbreviated Title:
Chinese Journal of Natural Medicines