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      Expression and Secretion of Transforming Growth Factor-β in Transformed and Nontransformed Retinal Pigment Epithelial Cells

      Ophthalmic Research

      S. Karger AG

      Transforming growth factor-β, Gene regulation, Protein secretion, Retinal pigment epithelialcells

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          Abstract

          The expression and secretion of different isoforms of transforming growth factor-β (TGFβ) were examined in cultured transformed and nontransformed human retinal pigment epithelial (RPE) cells. Transformed RPE cells were found to express high levels of TGFβ1 mRNA, low levels of TGFβ3 mRNA but no detectable TGFβ2 mRNA. If the cells were grown under serum-free conditions the expression of TGFβ increased. The mRNA expression was accompanied by secretion of TGFβ1 (but not TGFβ2) protein into the culture media. By comparison, nontransformed RPE cells were found to secrete similar amounts of TGFβ as transformed cells but predominantly secreted TGFβ2. The secretion of TGFβ from both transformed and nontransformed RPE cells increased if the cells were grown without serum. In conclusion, the results show that TGFβ is expressed and secreted by transformed and nontransformed human RPE cells and that this expression and secretion are regulated by the presence or absence of exogenous factors.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1994
          1994
          11 December 2009
          : 26
          : 6
          : 361-367
          Affiliations
          Department of Ophthalmology, Huddinge University Hospital and Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden
          Article
          267502 Ophthalmic Res 1994;26:361–367
          10.1159/000267502
          7715917
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 7
          Categories
          Original Paper

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