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      First genetically confirmed records of the little gulper shark Centrophorus uyato (Squaliformes: Centrophoridae) from Cypriot waters

      Author(s): , , , ,
      Publication date Created:
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      Journal: Biodiversity Data Journal
      Publisher: Pensoft Publishers

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          Abstract

          The taxonomy within the genus Centrophorus has been controversial almost since its origin, raising uncertainties about the identification, the phylogenetic placement and the geographical distribution of several species. The partial nucleotide sequences of two mitochondrial DNA gene regions, the cytochrome c oxidase subunit I and the 16S ribosomal RNA, genetically confirmed the presence of the little gulper shark in Cypriot waters. The species presence in the Mediterranean Sea is revised and discussed.

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          MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.

          Sudhir Kumar, Glen Stecher, Michael KaWah Li … (2018)
          The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine. Here, we report a transformation of Mega to enable cross-platform use on Microsoft Windows and Linux operating systems. Mega X does not require virtualization or emulation software and provides a uniform user experience across platforms. Mega X has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses. Mega X is available in two interfaces (graphical and command line) and can be downloaded from www.megasoftware.net free of charge.
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            Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data

            Matthew Kearse, Richard Moir, Amy Wilson … (2012)
            Summary: The two main functions of bioinformatics are the organization and analysis of biological data using computational resources. Geneious Basic has been designed to be an easy-to-use and flexible desktop software application framework for the organization and analysis of biological data, with a focus on molecular sequences and related data types. It integrates numerous industry-standard discovery analysis tools, with interactive visualizations to generate publication-ready images. One key contribution to researchers in the life sciences is the Geneious public application programming interface (API) that affords the ability to leverage the existing framework of the Geneious Basic software platform for virtually unlimited extension and customization. The result is an increase in the speed and quality of development of computation tools for the life sciences, due to the functionality and graphical user interface available to the developer through the public API. Geneious Basic represents an ideal platform for the bioinformatics community to leverage existing components and to integrate their own specific requirements for the discovery, analysis and visualization of biological data. Availability and implementation: Binaries and public API freely available for download at http://www.geneious.com/basic, implemented in Java and supported on Linux, Apple OSX and MS Windows. The software is also available from the Bio-Linux package repository at http://nebc.nerc.ac.uk/news/geneiousonbl. Contact: peter@biomatters.com
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              DNA barcoding Australia's fish species.

              Robert Ward, Tyler Zemlak, Bronwyn Innes … (2005)
              Two hundred and seven species of fish, mostly Australian marine fish, were sequenced (barcoded) for a 655 bp region of the mitochondrial cytochrome oxidase subunit I gene (cox1). Most species were represented by multiple specimens, and 754 sequences were generated. The GC content of the 143 species of teleosts was higher than the 61 species of sharks and rays (47.1% versus 42.2%), largely due to a higher GC content of codon position 3 in the former (41.1% versus 29.9%). Rays had higher GC than sharks (44.7% versus 41.0%), again largely due to higher GC in the 3rd codon position in the former (36.3% versus 26.8%). Average within-species, genus, family, order and class Kimura two parameter (K2P) distances were 0.39%, 9.93%, 15.46%, 22.18% and 23.27%, respectively. All species could be differentiated by their cox1 sequence, although single individuals of each of two species had haplotypes characteristic of a congener. Although DNA barcoding aims to develop species identification systems, some phylogenetic signal was apparent in the data. In the neighbour-joining tree for all 754 sequences, four major clusters were apparent: chimaerids, rays, sharks and teleosts. Species within genera invariably clustered, and generally so did genera within families. Three taxonomic groups-dogfishes of the genus Squalus, flatheads of the family Platycephalidae, and tunas of the genus Thunnus-were examined more closely. The clades revealed after bootstrapping generally corresponded well with expectations. Individuals from operational taxonomic units designated as Squalus species B through F formed individual clades, supporting morphological evidence for each of these being separate species. We conclude that cox1 sequencing, or 'barcoding', can be used to identify fish species.
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                Author and article information

                Contributors
                Vasiliki Kousteni: (View ORCID Profile)
                Marios Papageorgiou: (View ORCID Profile)
                Journal
                Title: Biodiversity Data Journal
                Abbreviated Title: BDJ
                Publisher: Pensoft Publishers
                ISSN (Electronic): 1314-2828
                ISSN (Print): 1314-2836
                Publication date Created: September 16 2021
                Publication date (Electronic): September 16 2021
                Volume: 9
                Article
                DOI: 10.3897/BDJ.9.e71837
                SO-VID: c8469d2f-87fe-4501-9850-9a1c932a3b9c
                Copyright © © 2021
                License:

                http://creativecommons.org/licenses/by/4.0/

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