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      Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata.

      Biotechnology Letters
      Ascomycota, chemistry, classification, metabolism, Biotransformation, Chromatography, Thin Layer, methods, Ginsenosides, Rhizopus, Species Specificity

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          Abstract

          Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ene (1), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ol (2), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (3), and 3-O-beta-D-glucopyranosyl-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.

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