Identification of constituents in Gui-Zhi-Jia-Ge-Gen-Tang by LC-IT-MS combined with LC-Q-TOF-MS and elucidation of their metabolic networks in rat plasma after oral administration
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Abstract
Gui-Zhi-Jia-Ge-Gen-Tang (GZJGGT) is a traditional Chinese medicine (TCM) prescription
commonly used to treat cervical spondylopathy, scapulohumeral periarthritis, etc
. Though it is widely applied in clinical practice, the effective constituents of
GZJGGT remain unclear. This was the first report on the identification of the chemical
constituents from GZJGGT in vitro and in vivo using LC-IT-MS combined with LC-Q-TOF-MS.
A total of 141 constituents were detected in GZJGGT, and 77 were identified. These
compounds mainly included flavonoid glycosides, triterpene saponins, monoterpene glycosides,
puerosides, and organic acids. Among them, 12 compounds were unequivocally identified
in comparison with reference substances. Additionally, a diagnostic base peak ion
filtering strategy for rapid classification of flavonoid O -glycosides and C -glycosides
was proposed. After gastrointestinal administration of GZJGGT to rats, 45 prototypes
and 48 metabolites in rat plasma were speculated. In addition, the metabolic profile
of GZJGGT was portrayed to understand interrelationship between metabolites.
Author and article information
Journal
Title:
Chinese Journal of Natural Medicines
Abbreviated Title:
Chinese Journal of Natural Medicines