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Abstract
Introduction
Extracellular heat shock proteins (HSP) act as inducers of interleukins (IL) and stimulants
for immune cells during systemic inflammatory response syndrome (SIRS). Little is
known about the alarming roles of extracellular HSP72 and HSP90α in the acute phase
[1] of sepsis (S) or severe sepsis (SS). We determined serum HSP90α, HSP72 and neutrophil
CD64 expression, IL-6, IL-8, IL-10, and TNFα in children with S or SS compared with
SIRS (brain injury) or healthy children (H).
Methods
Critically ill children with S (n = 16), SS (n = 15) or SIRS (n = 18) and H (n = 21)
were enrolled in the study. ELISA was used to evaluate HSPs, chemiluminescence to
measure ILs, and flow cytometry to evaluate nCD64 expression (IRB approved).
Results
Patients in both septic groups had elevated HSP90α (P < 0.0001), HSP72 (P < 0.05),
IL-6 (P < 0.0001), IL-8 (P < 0.02) and IL-10 (P < 0.05) levels compared with H, whereas
SS had increased HSP72, IL6 and TNFα compared with SIRS (P < 0.05). SIRS patients
presented increased HSP90α, IL-6 and IL-8 compared with H (P < 0.05). Both HSPs were
dramatically increased among nonsurvivors. In a logistic regression model, only HSP90α
was independently associated with mortality (P < 0.0001). HSP90α related positively
(P < 0.001) to nCD64, IL-8, IL-10, CRP, PRISM, PELOD, TISS, and LOS and negatively
to HDL (P < 0.001) and LDL (P < 0.02). HSP72 also related negatively to HDL (P < 0.001).
Conclusion
Extracellular HSP72 and HSP90α are alarmingly elevated in critically ill children,
especially in severe sepsis. HSP90α levels are independently associated with mortality,
related to CD64, IL-8, IL-10, severity of illness, and outcome. Both HSPs are inversely
related to the low LDL/low HDL septic metabolic pattern [2].
Acknowledgements
This research has been co-financed by the European Union (European Social Fund) and
Greek national funds through the Operational Program 'Education and Lifelong Learning'
of the National Strategic Reference Framework Research Funding Program: THALES. Investing
in knowledge society through the European Social Fund.
This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (
http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided
the original work is properly cited. The Creative Commons Public Domain Dedication
waiver (
http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Conference name:
35th International Symposium on Intensive Care and Emergency Medicine