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      Use of hare bone for the manufacture of a Clovis bead

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          Abstract

          A tubular bone bead dating to ~ 12,940 BP was recovered from a hearth-centered activity area at the La Prele Mammoth site in Converse County, Wyoming, USA. This is the oldest known bead from the Western Hemisphere. To determine the taxonomic origin of the bead, we extracted collagen for zooarchaeology by mass spectrometry (ZooMS). We also used micro-CT scanning for morphological analysis to determine likely skeletal elements used for its production. We conclude that the bead was made from a metapodial or proximal phalanx of a hare ( Lepus sp.). This find represents the first secure evidence for the use of hares during the Clovis period. While the use of hare bone for the manufacture of beads was a common practice in western North America during the Holocene, its origins can now be traced back to at least the terminal Pleistocene.

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          Most cited references51

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          Middle Stone Age shell beads from South Africa.

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            Cutmarks made by stone tools on bones from Olduvai Gorge, Tanzania

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              Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.

              Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip) for peptide purification, rather than liquid chromatography/mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10 degrees C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification. Copyright 2009 John Wiley & Sons, Ltd.
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                Author and article information

                Contributors
                surovell@uwyo.edu
                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group UK (London )
                2045-2322
                5 February 2024
                5 February 2024
                2024
                : 14
                : 2937
                Affiliations
                [1 ]Department of Anthropology, University of Wyoming, ( https://ror.org/01485tq96) Laramie, WY USA
                [2 ]GRID grid.446508.b, ISNI 0000 0001 2109 4315, Office of Archaeology and Historic Preservation, , History Colorado, ; Denver, CO USA
                [3 ]Manchester Institute of Biotechnology, The University of Manchester, ( https://ror.org/027m9bs27) Manchester, UK
                [4 ]Department of Molecular Biology, University of Wyoming, ( https://ror.org/01485tq96) Laramie, WY USA
                [5 ]Department of Anthropology, Chico State University, ( https://ror.org/027bzz146) Chico, CA USA
                [6 ]Office of the Wyoming State Archaeologist, Department of Anthropology, University of Wyoming, ( https://ror.org/01485tq96) Laramie, WY USA
                [7 ]Department of Sociology and Anthropology, Weber State University, ( https://ror.org/01epn2q93) Ogden, UT USA
                Article
                53390
                10.1038/s41598-024-53390-9
                10844228
                38316967
                02dd4f5e-6a44-460f-8637-7f2b2a30e1c4
                © The Author(s) 2024

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 23 October 2023
                : 31 January 2024
                Funding
                Funded by: National Science Foundation
                Award ID: 1947297
                Award ID: 1947297
                Award ID: 1947297
                Award ID: 1947297
                Award ID: 1947297
                Award Recipient :
                Funded by: FundRef http://dx.doi.org/10.13039/100006363, National Geographic Society;
                Award ID: 9896-16
                Award Recipient :
                Funded by: Wyoming Cultural Trust Fund
                Award ID: 212-17-S
                Award ID: 212-17-S
                Award Recipient :
                Funded by: Quest Archaeological Research Program
                Funded by: Roy J. Shlemon Center for Quaternary Studies
                Funded by: George C. Frison Institute of Archaeology and Anthropology
                Categories
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                Custom metadata
                © Springer Nature Limited 2024

                Uncategorized
                archaeology,proteomics
                Uncategorized
                archaeology, proteomics

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