Jaboticaba ( Myrciaria jaboticaba) peel extracts induce reticulum stress and apoptosis in breast cancer cells

Graphical abstract

Highlights

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  Ethyl acetate and water–ethanol extracts from Jaboticaba peel were assessed.

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  Both extracts inhibited the clonogenic potential of MDA-MB-231 breast cancer cells.

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  Ethyl acetate extract was more effective against MCF7 cells.

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  Extracts’ action mechanisms can be the induction of endoplasmic stress and apoptosis.

Abstract

Jaboticaba peel ( Myrciaria jaboticaba) is a source of bioactive compounds. We investigated the anticancer activity of ethyl acetate extract (JE1) and hydroethanolic extract (JE2) of Jaboticaba peel against breast cancer. Both JE1 and JE2 inhibited clonogenic potential of MDA-MB-231 cells while JE1 was particularly effective in MCF7 cells. Anchorage-independent growth and cell viability was also inhibited by JE1 and JE2. In addition to growth inhibition, JE1 and JE2 could also inhibit migration and invasion of cells. Interestingly, JE1 and JE2 show selective inhibition towards certain breast cancer cells and biological processes. Mechanistic evaluations showed that JE1 induced PARP cleavage, BAX and BIP indicating apoptotic induction. An elevation of phosphorylated ERK was observed in MCF7 cells in response to JE1 and JE2 along with increased IRE-α and CHOP expression indicating increased endoplasmic stress. Therefore, Jaboticaba peel extracts could be potentially considered for further development for breast cancer inhibition.